標題: Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments in a Single Cell
作者: Nakabayashi, Takakazu
Awasthi, Kamlesh
Ohta, Nobuhiro
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Fluorescence lifetime imaging;TCSPC;Femtosecond laser;Intracellular pH;Intracellular environment;Ratiometric method
公開日期: 1-Jan-2017
摘要: Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images requires an understanding of the theory of fluorescence lifetime, principle of FLIM systems, and evaluation procedure of intracellular environments. In this chapter, the materials, methods, and notes on FLIM measurements have been described, in conjunction with a brief explanation of the background of FLIM.
URI: http://dx.doi.org/10.1007/978-3-319-67358-5_8
http://hdl.handle.net/11536/145247
ISSN: 0065-2598
DOI: 10.1007/978-3-319-67358-5_8
期刊: MULTI-PARAMETRIC LIVE CELL MICROSCOPY OF 3D TISSUE MODELS
Volume: 1035
起始頁: 121
結束頁: 133
Appears in Collections:Articles