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dc.contributor.authorHung, Chih-Changen_US
dc.contributor.authorChen, Xiao-Ruen_US
dc.contributor.authorKo, Ying-Kuanen_US
dc.contributor.authorKobayashi, Takayoshien_US
dc.contributor.authorYang, Chii-Shenen_US
dc.contributor.authorYabushita, Atsushien_US
dc.date.accessioned2018-08-21T05:54:13Z-
dc.date.available2018-08-21T05:54:13Z-
dc.date.issued2017-06-20en_US
dc.identifier.issn0006-3495en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bpj.2017.05.015en_US
dc.identifier.urihttp://hdl.handle.net/11536/145685-
dc.description.abstractIn this study, we investigated the ultrafast dynamics of bacteriorhodopsins (BRs) from Haloquadratum walsbyi (HwBR) and Haloarcula marismortui (HmBRI and HmBRII). First, the ultrafast dynamics were studied for three HwBR samples: wild-type, D93N mutation, and D104N mutation. The residues of the D93 and D104 mutants correspond to the control by the Schiff base proton acceptor and donor of the proton translocation subchannels. Measurements indicated that the negative charge from the Schiff base proton acceptor residue D93 interacts with the ultrafast and substantial change of the electrostatic potential associated with chromophore isomerization. By contrast, the Schiff base proton donor assists the restructuring of the chromophore cavity hydrogen-bond network during the thermalization of the vibrational hot state. Second, the ultrafast dynamics of the wild-types of HwBR, HmBRI, and HmBRII were compared. Measurements demonstrated that the hydrogen-bond network in the extracellular region in HwBR and HmBRII slows the photoisomerization of retinal chromophores, and the negatively charged helices on the cytoplasmic side of HwBR and HmBRII accelerate the thermalization of the vibrational hot state of retinal chromophores. The similarity of the correlation spectra of the wild-type HmBRI and D104N mutant of HwBR indicates that inactivation of the Schiff base proton donor induces a positive charge on the helices of the cytoplasmic side.en_US
dc.language.isoen_USen_US
dc.titleSchiff Base Proton Acceptor Assists Photoisomerization of Retinal Chromophores in Bacteriorhodopsinen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bpj.2017.05.015en_US
dc.identifier.journalBIOPHYSICAL JOURNALen_US
dc.citation.volume112en_US
dc.citation.spage2503en_US
dc.citation.epage2519en_US
dc.contributor.department電子物理學系zh_TW
dc.contributor.departmentDepartment of Electrophysicsen_US
dc.identifier.wosnumberWOS:000404078700011en_US
Appears in Collections:Articles