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dc.contributor.authorTu, Chang-Chingen_US
dc.contributor.authorAwasthi, Kamleshen_US
dc.contributor.authorChen, Kuang-Poen_US
dc.contributor.authorLin, Chih-Hsiangen_US
dc.contributor.authorHamada, Morihikoen_US
dc.contributor.authorOhta, Nobuhiroen_US
dc.contributor.authorLi, Yaw-Kuenen_US
dc.date.accessioned2018-08-21T05:54:13Z-
dc.date.available2018-08-21T05:54:13Z-
dc.date.issued2017-06-01en_US
dc.identifier.issn2330-4022en_US
dc.identifier.urihttp://dx.doi.org/10.1021/acsphotonics.7b00188en_US
dc.identifier.urihttp://hdl.handle.net/11536/145688-
dc.description.abstractIn this work we demonstrate time-gated confocal fluorescence imaging on live cancer cells immunostained by antibody-conjugated silicon quantum dot nano particles (SiQD-NPs) and organic dyes, for simultaneous detection of two biological targets and removal of background autofluorescence. With almost all radiative recombinations occurring through oxide-related defect states located on the SiQD surface, the SiQD-NPs have very long photoluminescence lifetimes of about 25 its, in contrast to the nanosecond range lifetimes of other commonly used biological fluorophores. This drastic lifetime difference enables a time-gated imaging method here, in which the time-resolved photon distribution of each pixel of a fluorescence image is measured by using a time-correlated single-photon counting technique. Then, by integrating the photon histogram of each pixel over respective time windows, the long-lived component of the fluorescence image comprising only the fluorescence emitted from the SiQD-NPs is separated from all other short-lived signals resulting from the organic dyes and the cell endogenous luminescence. For instance, the membrane and nucleus of a single cancer cell or two types of cancer cells, immunostained with the SiQD-NPs and the organic dyes, respectively, can be clearly distinguished from each other by time-gating, which otherwise cannot be accomplished by conventional multiplexing due to spectral overlap in the wavelength domain.en_US
dc.language.isoen_USen_US
dc.subjectsilicon quantum dotsen_US
dc.subjecttime-gated imagingen_US
dc.subjectimmunofluorescenceen_US
dc.subjectbioconjugationen_US
dc.subjectbiological labelsen_US
dc.titleTime-Gated Imaging on Live Cancer Cells Using Silicon Quantum Dot Nanoparticles with Long-Lived Fluorescenceen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/acsphotonics.7b00188en_US
dc.identifier.journalACS PHOTONICSen_US
dc.citation.volume4en_US
dc.citation.spage1306en_US
dc.citation.epage1315en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000404098200003en_US
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