標題: | Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
作者: | Fang, Chih-Hsiang Tsai, Pei-I Huang, Shu-Wei Sun, Jui-Sheng Chang, Jenny Zwei-Chieng Shen, Hsin-Hsin Chen, San-Yuan Lin, Feng Huei Hsu, Lih-Tao Chen, Yen-Chun 材料科學與工程學系 智慧型仿生系統研究中心 Department of Materials Science and Engineering Biomimetic Systems Research Center |
關鍵字: | Peri-implant osteomyelitis;Magnetic nanoparticle;Hyperthermia;Biofilm |
公開日期: | 25-七月-2017 |
摘要: | Background: When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis. Methods: Sixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed. Results: The pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 degrees C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control. Conclusions: For the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced. |
URI: | http://dx.doi.org/10.1186/s12879-017-2621-4 http://hdl.handle.net/11536/145836 |
ISSN: | 1471-2334 |
DOI: | 10.1186/s12879-017-2621-4 |
期刊: | BMC INFECTIOUS DISEASES |
Volume: | 17 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 期刊論文 |