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dc.contributor.authorKung, Wei-Hsiangen_US
dc.contributor.authorYu, Ching-Fangen_US
dc.contributor.authorLee, Andy Chi-Lungen_US
dc.contributor.authorYang, Chi-Dungen_US
dc.contributor.authorLiu, Yu-Chenen_US
dc.contributor.authorChen, Fang-Hsinen_US
dc.contributor.authorHuang, Hsien-Daen_US
dc.date.accessioned2018-08-21T05:54:24Z-
dc.date.available2018-08-21T05:54:24Z-
dc.date.issued2017-08-01en_US
dc.identifier.issn1476-9271en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.compbiolchem.2017.04.010en_US
dc.identifier.urihttp://hdl.handle.net/11536/145897-
dc.description.abstractRadiotherapy (RT) is a common cancer treatment approach that accounts for nearly 50% of patient treatment; however, tumor relapse after radiotherapy is still a major issue. To study the crucial role of tumor-associated macrophages (TAMs) in the regulation of tumor progression post-RT, microarray experiments comparing TAM gene expression profiles between unirradiated and irradiated tumors were conducted to discover possible roles of TAMs in initiation or contribution to tumor recurrence following RT, taking into account the relationships among gene expression, tumor microenvironment, and immunology. A single dose of 25 Gy was given to TRAMP C-1 prostate tumors established in C57/B6 mice. CD11b-positive macrophages were extracted from the tumors at one, two and three weeks post-RT. Gene ontology (GO) term analysis using the DAVID database revealed that genes that were differentially expressed at one and two weeks after irradiation were associated with biological processes such as morphogenesis of a branching structure, tube development, and cell proliferation. Analysis using Short Time-Series Expression Miner (STEM) revealed the temporal gene expression profiles and identified 13 significant patterns in four main groups of profiles. The genes in the upregulated temporal profile have diverse functions involved in the intracellular signaling cascade, cell proliferation, and cytokinemediated signaling pathway. We show that tumor irradiation with a single 25-Gy dose can initiate a time series of differentially expressed genes in TAMs, which are associated with the immune response, DNA repair, cell cycle arrest, and apoptosis. Our study helps to improve our understanding of the function of the group of genes whose expression changes temporally in an irradiated tumor microenvironment. (C) 2017 Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectTumor-associated macrophagesen_US
dc.subjectTumor microenvironmenten_US
dc.subjectMicroarrayen_US
dc.subjectTemporal gene expressionen_US
dc.titleGene expression profiling of tumor-associated macrophages after exposure to single-dose irradiationen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.compbiolchem.2017.04.010en_US
dc.identifier.journalCOMPUTATIONAL BIOLOGY AND CHEMISTRYen_US
dc.citation.volume69en_US
dc.citation.spage138en_US
dc.citation.epage146en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.identifier.wosnumberWOS:000407403300016en_US
Appears in Collections:Articles