完整後設資料紀錄
DC 欄位語言
dc.contributor.authorLo, Tiffany Chien-Tingen_US
dc.contributor.authorHsu, Feng-Mingen_US
dc.contributor.authorChang, C. Allenen_US
dc.contributor.authorCheng, Jason Chia-Hseinen_US
dc.date.accessioned2014-12-08T15:20:33Z-
dc.date.available2014-12-08T15:20:33Z-
dc.date.issued2011-11-23en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://dx.doi.org/10.1021/jf202457ren_US
dc.identifier.urihttp://hdl.handle.net/11536/14646-
dc.description.abstractThis work investigated the role of structure in the binding of polysaccharides from 10 regionally different strains of Lentinula edodes to Toll-like receptor 4 (TLR-4) on monocytes (THP-1) and the potential effect of this interaction on tumor cell viability. Principal component analysis and multiple linear regression identified arabinose, glucose 1 -> 4 linkage, and molecular weights about 2700 and 534 kDa as the significant determinant factors associated with TLR-4 binding activity. The branched alpha-(1,4)-glucan (L10) had the strongest ability to bind to TLR-4 and induce THP-1 cell differentiation. L10 induction of the THP-1 cell differentiation, superoxide production, and cytokine production followed the TLR-4/MyD88/IKK/NF kappa B pathway. Coculture of irradiated human lung adenocarcinoma A549 cells with L10-activated THP-1 cells resulted in significantly decreased percentage of viable A549 cells from 66 to 37% (p = 0.018), increased levels of superoxide, interleukin-8, and RANTES, and decreased levels of angiogenin and vascular endothelial growth factor. The results indicate that L10-activated monocytes have the potential to boost the antitumor immune response and antitumor activity of radiotherapy.en_US
dc.language.isoen_USen_US
dc.subjectpolysaccharideen_US
dc.subjectmultiple linear regression analysisen_US
dc.subjectmacrophage differentiation/activationen_US
dc.subjectimmunotherapyen_US
dc.subjectradiation therapyen_US
dc.titleBranched alpha-(1,4) Glucans from Lentinula edodes (L10) in Combination with Radiation Enhance Cytotoxic Effect on Human Lung Adenocarcinoma through the Toll-like Receptor 4 Mediated Induction of THP-1 Differentiation/Activationen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/jf202457ren_US
dc.identifier.journalJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRYen_US
dc.citation.volume59en_US
dc.citation.issue22en_US
dc.citation.spage11997en_US
dc.citation.epage12005en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000296992800014-
dc.citation.woscount3-
顯示於類別:期刊論文


文件中的檔案:

  1. 000296992800014.pdf

若為 zip 檔案,請下載檔案解壓縮後,用瀏覽器開啟資料夾中的 index.html 瀏覽全文。