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dc.contributor.authorCarone, Benjamin R.en_US
dc.contributor.authorHung, Jui-Hungen_US
dc.contributor.authorHainer, Sarah J.en_US
dc.contributor.authorChou, Min-Teen_US
dc.contributor.authorCarone, Dawn M.en_US
dc.contributor.authorWeng, Zhipingen_US
dc.contributor.authorFazzio, Thomas G.en_US
dc.contributor.authorRando, Oliver J.en_US
dc.date.accessioned2019-04-02T06:00:44Z-
dc.date.available2019-04-02T06:00:44Z-
dc.date.issued2014-07-01en_US
dc.identifier.issn1534-5807en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.devcel.2014.05.024en_US
dc.identifier.urihttp://hdl.handle.net/11536/147749-
dc.description.abstractMammalian embryonic stem cells (ESCs) and sperm exhibit unusual chromatin packaging that plays important roles in cellular function. Here, we extend a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-binding proteins genome-wide in murine ESCs and sperm. In ESCs, we recover well-characterized features of chromatin such as promoter nucleosome depletion and further identify widespread footprints of sequence-specific DNA-binding proteins such as CTCF, which we validate in knockdown studies. We document global differences in nuclease accessibility between ESCs and sperm, finding that the majority of histone retention in sperm preferentially occurs in large gene-poor genomic regions, with only a small subset of nucleosomes being retained over promoters of developmental regulators. Finally, we describe evidence that CTCF remains associated with the genome in mature sperm, where it could play a role in organizing the sperm genome.en_US
dc.language.isoen_USen_US
dc.titleHigh-Resolution Mapping of Chromatin Packaging in Mouse Embryonic Stem Cells and Spermen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.devcel.2014.05.024en_US
dc.identifier.journalDEVELOPMENTAL CELLen_US
dc.citation.volume30en_US
dc.citation.spage11en_US
dc.citation.epage22en_US
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.identifier.wosnumberWOS:000339307400006en_US
dc.citation.woscount95en_US
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