完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chen, Yi-Jou | en_US |
dc.contributor.author | Chen, Michael | en_US |
dc.contributor.author | Hsieh, Yuan-Chin | en_US |
dc.contributor.author | Su, Yu-Cheng | en_US |
dc.contributor.author | Wang, Chang-Hung | en_US |
dc.contributor.author | Cheng, Chiu-Min | en_US |
dc.contributor.author | Kao, An-Pei | en_US |
dc.contributor.author | Wang, Kai-Hung | en_US |
dc.contributor.author | Cheng, Jing-Jy | en_US |
dc.contributor.author | Chuang, Kuo-Hsiang | en_US |
dc.date.accessioned | 2019-04-02T05:58:26Z | - |
dc.date.available | 2019-04-02T05:58:26Z | - |
dc.date.issued | 2018-12-14 | en_US |
dc.identifier.issn | 2045-2322 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1038/s41598-018-36192-8 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/148597 | - |
dc.description.abstract | The sensitivity of traditional enzyme-linked immunosorbent assays (ELISAs) is limited by the low binding avidity and heterogeneous orientation of capture antibodies coated on polystyrene-based microplates. Here, we developed a highly sensitive ELISA strategy by fixing poly-protein G-expressing cells on microplates to improve the coating amount and displayed orientation of capture antibodies. One or eight repeated fragment crystallisable (Fc) binding domains of protein G are stably expressed on the surface of BALB/c 3T3 cells (termed 1pG cells or 8pG cells), which then act as highly antibody-trapping microparticles. The 8pG cells showed higher antibody-trapping ability than the 1pG cells did. The antibody-coating amount of the 8pG cell-based microplates was 1.5-23 times and 1.2-6.8 times higher than that of traditional polystyrene-based and commercial protein G-based microplates, respectively. The 8pG cell-based microplates were then applied to an anti-IFN-alpha sandwich ELISA and an anti-CTLA4 competitive ELISA, respectively, and dramatically enhanced their detection sensitivity. Importantly, direct coating unpurified capture antibody produced by mammalian cells did not impair the antigen-capturing function of 8pG cell-based microplates. The 8pG cell-based microplates exhibited a significant improvement in antibody-coating amount and preserved the homogeneous orientation of capture antibodies, making them a potential replacement for traditional microplates in various formats of ELISAs. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of polyprotein G-expressing cell-based microplates | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1038/s41598-018-36192-8 | en_US |
dc.identifier.journal | SCIENTIFIC REPORTS | en_US |
dc.citation.volume | 8 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000453224400029 | en_US |
dc.citation.woscount | 0 | en_US |
顯示於類別: | 期刊論文 |