標題: Gut microbiome changes in overweight male adults following bowel preparation
作者: Chen, Hui-Mei
Chen, Chung-Chu
Chen, Chien-Chi
Wang, Shen-Chih
Wang, Chun-Lin
Huang, Chien-Hsun
Liou, Jong-Shian
Liu, Ta-Wei
Peng, Hwei-Ling
Lin, Feng-Mao
Liu, Chia-Yuan
Weng, Shun-Long
Cheng, Chieh-Jen
Hung, Yi-Fang
Liao, Chii-Cherng
Huang, Hsien-Da
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
關鍵字: Gut microbiome;Overweight;Bowel preparation;High-throughput sequencing;Bacteroides;Preovotella
公開日期: 31-十二月-2018
摘要: BackgroundHuman gut microbiome has an essential role in human health and disease. Although the major dominant microbiota within individuals have been reported, the change of gut microbiome caused by external factors, such as antibiotic use and bowel cleansing, remains unclear. We conducted this study to investigate the change of gut microbiome in overweight male adults after bowel preparation, where none of the participants had been diagnosed with any systemic diseases.MethodsA total of 20 overweight, male Taiwanese adults were recruited, and all participants were omnivorous. The participants provided fecal samples and blood samples at three time points: prior to bowel preparation, 7days after colonoscopy, and 28days after colonoscopy. The microbiota composition in fecal samples was analyzed using 16S ribosome RNA gene amplicon sequencing.ResultsOur results demonstrated that the relative abundance of the most dominant bacteria hardly changed from prior to bowel preparation to 28days after colonoscopy. Using the ratio of Prevotella to the sum of Prevotella and Bacteroides in the fecal samples at baseline, the participants were separated into two groups. The fecal samples of the Type 1 group was Bacteroides-dominant, and that of the Type 2 group was Prevotella-dominant with a noticeable presence Bacteroides. Bulleidia appears more in the Type 1 fecal samples, while Akkermensia appears more in the Type 2 fecal samples. Of each type, the gut microbial diversity differed slightly among the three collection times. Additionally, the Type 2 fecal microbiota was temporarily susceptible to bowel cleansing. Predictive functional analysis of microbial community reveals that their activities for the mineral absorption metabolism and arachidonic acid metabolism differed significantly between the two types. Depending on their fecal type, the variance of triglycerides and C-reactive protein also differed between the two types of participants.ConclusionsDepending upon the fecal type, the microbial diversity and the predictive functional modules of microbial community differed significantly after bowel preparation. In addition, blood biochemical markers presented somewhat associated with fecal type. Therefore, our results might provide some insights as to how knowledge of the microbial community could be used to promote health through personalized clinical treatment.
URI: http://dx.doi.org/10.1186/s12864-018-5285-6
http://hdl.handle.net/11536/148655
ISSN: 1471-2164
DOI: 10.1186/s12864-018-5285-6
期刊: BMC GENOMICS
Volume: 19
Issue: 10
顯示於類別:期刊論文