標題: Idi1 and Hmgcs2 Are Affected by Stretch in HL-1 Atrial Myocytes
作者: Fang, Chih-Yuan
Chen, Mien-Cheng
Chang, Tzu-Hao
Wu, Chia-Chen
Chang, Jen-Ping
Huang, Hsien-Da
Ho, Wan-Chun
Wang, Yi-Zhen
Pan, Kuo-Li
Lin, Yu-Sheng
Huang, Yao-Kuang
Chen, Chien-Jen
Lee, Wei-Chieh
生物資訊及系統生物研究所
Institude of Bioinformatics and Systems Biology
關鍵字: atrium;genes;lipid;myopathy
公開日期: 1-十二月-2018
摘要: Background: Lipid expression is increased in the atrial myocytes of mitral regurgitation (MR) patients. This study aimed to investigate key regulatory genes and mechanisms of atrial lipotoxic myopathy in MR. Methods: The HL-1 atrial myocytes were subjected to uniaxial cyclic stretching for eight hours. Fatty acid metabolism, lipoprotein signaling, and cholesterol metabolism were analyzed by PCR assay (168 genes). Results: The stretched myocytes had significantly larger cell size and higher lipid expression than non-stretched myocytes (all p < 0.001). Fatty acid metabolism, lipoprotein signaling, and cholesterol metabolism in the myocytes were analyzed by PCR assay (168 genes). In comparison with their counterparts in non-stretched myocytes, seven genes in stretched monocytes (Idi1, Olr1, Nr1h4, Fabp2, Prkag3, Slc27a5, Fabp6) revealed differential upregulation with an altered fold change >1.5. Nine genes in stretched monocytes (Apoa4, Hmgcs2, Apol8, Srebf1, Acsm4, Fabp1, Acox2, Acsl6, Gk) revealed differential downregulation with an altered fold change <0.67. Canonical pathway analysis, using Ingenuity Pathway Analysis software, revealed that the only genes in the superpathway of cholesterol biosynthesis were Idi1 (upregulated) and Hmgcs2 (downregulated). The fraction of stretched myocytes expressing Nile red was significantly decreased by RNA interference of Idi1 (p < 0.05) and was significantly decreased by plasmid transfection of Hmgcs2 (p = 0.004). Conclusions: The Idi1 and Hmgcs2 genes have regulatory roles in atrial lipotoxic myopathy associated with atrial enlargement.
URI: http://dx.doi.org/10.3390/ijms19124094
http://hdl.handle.net/11536/148702
ISSN: 1422-0067
DOI: 10.3390/ijms19124094
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 19
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