標題: FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H2O2/H2S formation through multicolor images
作者: Velusamy, Nithya
Thirumalaivasan, Natesan
Bobba, Kondapa Naidu
Podder, Arup
Wu, Shu-Pao
Bhuniya, Sankarprasad
應用化學系
Department of Applied Chemistry
關鍵字: FRET- based fluorescent probe;Coumarin;Naphthalimide;Endogenous H2O2;Endogenous H2S
公開日期: 1-Feb-2019
摘要: We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H2S and H2O2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV-Vis absorption (lambda(abs) 390 nm) and fluorescence emission (lambda(em) 460 nm) of probe PHS1 in the presence of H2O2 are increased similar to 35-fold and similar to 15-fold respectively. It also allows the estimation of the levels of H2S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4-9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H2O2, H2S, and H2O2/H2S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H2S, H2O2) in cancer cells.
URI: http://dx.doi.org/10.1016/j.jphotobiol.2018.12.016
http://hdl.handle.net/11536/148875
ISSN: 1011-1344
DOI: 10.1016/j.jphotobiol.2018.12.016
期刊: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume: 191
起始頁: 99
結束頁: 106
Appears in Collections:Articles