標題: | Lipo-PEG-PEI complex as an intracellular transporter for protein therapeutics |
作者: | Lin, Yu-Ling Chen, Chia-Hung Liu, Yen-Ku Huang, Tse-Hung Tsai, Nu-Man Tzou, Shey-Cherng Liao, Kuang-Wen 交大名義發表 生物科技學系 分子醫學與生物工程研究所 National Chiao Tung University Department of Biological Science and Technology Institute of Molecular Medicine and Bioengineering |
關鍵字: | liposomal polyethylenimine and polyethylene glycol complex;LPPC;intracellular protein delivery;endocytosis;enhanced green fluorescent protein;EGFP;beta-glucuronidase |
公開日期: | 1-一月-2019 |
摘要: | Background: Protein or peptide drugs are emerging therapeutics for treating human diseases. However, current protein drugs are typically limited to acting on extracellular/cell membrane components associated with the diseases, while intracellular delivery of recombinant proteins replaces or replenishes faulty/missing proteins and remains inadequate. In this study, we developed a convenient and efficient intracellular protein delivery vehicle. Materials and methods: A cationic liposomal polyethylenimine and polyethylene glycol complex (LPPC) was developed to noncovalently capture proteins for protein transfer into cells via endocytosis. beta-glucuronidase (beta G) was used in vitro and in vivo as a model enzyme to demonstrate the enzymatic activity of the intracellular transport of a protein. Results: The endocytosed protein/LPPC complexes escaped from lysosomes, and the bound protein dissociated from LPPC in the cytosol. The enzymatic activity of beta G was well preserved after intracellular delivery in vitro and in vivo. Conclusion: Using LPPC as an intracellular protein transporter for protein therapeutics, we illustrated that LPPC may be an effective and convenient tool for studying diseases and developing therapeutics. |
URI: | http://dx.doi.org/10.2147/IJN.S188970 http://hdl.handle.net/11536/148886 |
ISSN: | 1178-2013 |
DOI: | 10.2147/IJN.S188970 |
期刊: | INTERNATIONAL JOURNAL OF NANOMEDICINE |
Volume: | 14 |
起始頁: | 1119 |
結束頁: | 1130 |
顯示於類別: | 期刊論文 |