標題: Lipo-PEG-PEI complex as an intracellular transporter for protein therapeutics
作者: Lin, Yu-Ling
Chen, Chia-Hung
Liu, Yen-Ku
Huang, Tse-Hung
Tsai, Nu-Man
Tzou, Shey-Cherng
Liao, Kuang-Wen
交大名義發表
生物科技學系
分子醫學與生物工程研究所
National Chiao Tung University
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: liposomal polyethylenimine and polyethylene glycol complex;LPPC;intracellular protein delivery;endocytosis;enhanced green fluorescent protein;EGFP;beta-glucuronidase
公開日期: 1-一月-2019
摘要: Background: Protein or peptide drugs are emerging therapeutics for treating human diseases. However, current protein drugs are typically limited to acting on extracellular/cell membrane components associated with the diseases, while intracellular delivery of recombinant proteins replaces or replenishes faulty/missing proteins and remains inadequate. In this study, we developed a convenient and efficient intracellular protein delivery vehicle. Materials and methods: A cationic liposomal polyethylenimine and polyethylene glycol complex (LPPC) was developed to noncovalently capture proteins for protein transfer into cells via endocytosis. beta-glucuronidase (beta G) was used in vitro and in vivo as a model enzyme to demonstrate the enzymatic activity of the intracellular transport of a protein. Results: The endocytosed protein/LPPC complexes escaped from lysosomes, and the bound protein dissociated from LPPC in the cytosol. The enzymatic activity of beta G was well preserved after intracellular delivery in vitro and in vivo. Conclusion: Using LPPC as an intracellular protein transporter for protein therapeutics, we illustrated that LPPC may be an effective and convenient tool for studying diseases and developing therapeutics.
URI: http://dx.doi.org/10.2147/IJN.S188970
http://hdl.handle.net/11536/148886
ISSN: 1178-2013
DOI: 10.2147/IJN.S188970
期刊: INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume: 14
起始頁: 1119
結束頁: 1130
顯示於類別:期刊論文