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dc.contributor.authorZhang, Xiao-Yanen_US
dc.contributor.authorLi, Zhe-Yuen_US
dc.contributor.authorZhang, Yuen_US
dc.contributor.authorZang, Xiao-Qianen_US
dc.contributor.authorUeno, Koseien_US
dc.contributor.authorMisawa, Hiroakien_US
dc.contributor.authorSun, Kaien_US
dc.date.accessioned2019-04-02T05:58:19Z-
dc.date.available2019-04-02T05:58:19Z-
dc.date.issued2019-01-01en_US
dc.identifier.issn2072-666Xen_US
dc.identifier.urihttp://dx.doi.org/10.3390/mi10010055en_US
dc.identifier.urihttp://hdl.handle.net/11536/148929-
dc.description.abstractCapacitively coupled contactless conductivity detection ((CD)-D-4) is an improved approach to avoid the problems of labor-intensive, time-consuming and insufficient accuracy of plate count as well as the high-cost apparatus of flow cytometry (FCM) in bacterial counting. This article describes a novel electrode-integrated printed-circuit-board (PCB)-based (CD)-D-4 device, which supports the simple and safe exchange of capillaries and improves the sensitivity and repeatability of the contactless detection. Furthermore, no syringe pump is needed in the detection, it reduces the system size, and, more importantly, avoids the effect on the bacteria due to high pressure. The recovered bacteria after (CD)-D-4 detection at excitation of 25 Vpp and 60-120 kHz were analyzed by flow cytometry, and a survival rate higher than 96% was given. It was verified that (CD)-D-4 detection did not influence the bacterial viability. Moreover, bacteria concentrations from 10(6) cells/mL to 10(8) cells/mL were measured in a linear range, and relative standard deviation (RSD) is below 0.2%. In addition, the effects on bacteria and (CD)-D-4 from background solutions were discussed. In contrast to common methods used in most laboratories, this method may provide a simple solution to in situ detection of bacterial cultures.en_US
dc.language.isoen_USen_US
dc.subjectbacterial concentrationen_US
dc.subjectcapacitively coupled contactless conductivity detection ((CD)-D-4)en_US
dc.subjectcapillaryen_US
dc.subjectEen_US
dc.subjectcolien_US
dc.subjectprinted-circuit-board (PCB)en_US
dc.titleBacterial Concentration Detection using a PCB-based Contactless Conductivity Sensoren_US
dc.typeArticleen_US
dc.identifier.doi10.3390/mi10010055en_US
dc.identifier.journalMICROMACHINESen_US
dc.citation.volume10en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000459735300054en_US
dc.citation.woscount0en_US
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