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dc.contributor.authorHuang, CPen_US
dc.contributor.authorLiu, HWen_US
dc.contributor.authorTsao, CYen_US
dc.contributor.authorYin, LTen_US
dc.contributor.authorChiu, SFen_US
dc.contributor.authorChen, TMen_US
dc.date.accessioned2019-04-02T06:00:18Z-
dc.date.available2019-04-02T06:00:18Z-
dc.date.issued2005-07-22en_US
dc.identifier.issn0925-4005en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.snb.2004.11.051en_US
dc.identifier.urihttp://hdl.handle.net/11536/148980-
dc.description.abstractA biochemical assay based on fluorescence imaging analysis by confocal laser scanning as a detection technique is specified. The method involved a glass plate coated with nitrocellulose (NC), where alpha-human-IgE-biotin at various concentrations was immobilized on an NC plate. Luminescent core-shell CdSe/ZnS QD-SA conjugates were then used as a fluorescent labeling agent to be captured specifically by biotinated alpha-human-IgE immobilized in a micro-array by affinity binding between SA and biotin in a plate-based biochemical assay. A confocal laser scanner was used to detect the fluorescence signals from the alpha-human-IgE-biotin-SA-QD complex. Experimental findings reveal that fluorescence intensity of QD-SA saturated at QD concentrations above 0.4 mg/ml. Moreover, a calibration curve between the fluorescence intensity and the concentration of alpha-human-IgE-biotin is plotted. The range of detectable concentrations of biotinlated alpha-human IgE was found to be between 3.3 and 100 mu g/ml in the a-human-IgE-biotin-SA-QD complex. Therefore, the results were consistent with the binding specificity in a plate-based biochemical assay, as determined using luminescent QDs as a labeling agent. (c) 2004 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectquantum dots (QD)en_US
dc.subjectstreptavidin (SA)en_US
dc.subjectfluorescenceen_US
dc.subjectbiochemical assayen_US
dc.titlePlate-based biochemical assay using quantum dots as a fluorescent labeling agenten_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.snb.2004.11.051en_US
dc.identifier.journalSENSORS AND ACTUATORS B-CHEMICALen_US
dc.citation.volume108en_US
dc.citation.spage713en_US
dc.citation.epage720en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000230330300118en_US
dc.citation.woscount12en_US
Appears in Collections:Articles