標題: Application of Data-Independent Acquisition Approach to Study the Proteome Change from Early to Later Phases of Tomato Pathogenesis Responses
作者: Fan, Kai-Ting
Wang, Kuo-Hsin
Chang, Wei-Hung
Yang, Jhih-Ci
Yeh, Ching-Fang
Cheng, Kai-Tan
Hung, Sheng-Chi
Chen, Yet-Ran
交大名義發表
National Chiao Tung University
關鍵字: plant pathogenesis responses;data-independent acquisition;quantitative proteomics;Pseudomonas syringae
公開日期: 2-二月-2019
摘要: Plants and pathogens are entangled in a continual arms race. Plants have evolved dynamic defence and immune mechanisms to resist infection and enhance immunity for second wave attacks from the same or different types of pathogenic species. In addition to evolutionarily and physiological changes, plant-pathogen interaction is also highly dynamic at the molecular level. Recently, an emerging quantitative mass spectrometry-based proteomics approach named data-independent acquisition (DIA), has been developed for the analysis of the proteome in a high-throughput fashion. In this study, the DIA approach was applied to quantitatively trace the change in the plant proteome from the early to the later stage of pathogenesis progression. This study revealed that at the early stage of the pathogenesis response, proteins directly related to the chaperon were regulated for the defence proteins. At the later stage, not only the defence proteins but also a set of the pathogen-associated molecular pattern-triggered immunity (PTI) and effector triggered immunity (ETI)-related proteins were highly induced. Our findings show the dynamics of the plant regulation of pathogenesis at the protein level and demonstrate the potential of using the DIA approach for tracing the dynamics of the plant proteome during pathogenesis responses.
URI: http://dx.doi.org/10.3390/ijms20040863
http://hdl.handle.net/11536/148999
ISSN: 1422-0067
DOI: 10.3390/ijms20040863
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 20
顯示於類別:期刊論文