標題: | Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging |
作者: | Wu, Chi-Ming Chen, Yen-Hao Dayananda, Kasala Shiue, Tsun-Wei Hung, Chen-Hsiung Liaw, Wen-Feng Chen, Po-Yu Wang, Yun-Ming 生物科技學系 Department of Biological Science and Technology |
關鍵字: | Nitric oxide;Fluorescent probe;Cell imaging;Rhodamine |
公開日期: | 5-十二月-2011 |
摘要: | A colorless and non-fluorescent rhodamine derivative, rhodamine B hydrazide (RH), is applied to detect nitric oxide and form fluorescent rhodamine B (RB). The reaction mechanism of RH with NO is proposed in this study. The probe shows good stability over a broad pH range (pH > 4). Furthermore, fluorescence intensity of RH displays an excellent linearity to the NO concentration and the detection limit is as low as 20 nM. A 1000-fold fluorescence turn-on from a dark background was observed. Moreover, the selectivity study indicated that the fluorescence intensity increasing in the presence of NO was significantly higher than those of other reactive oxygen/nitrogen species. In exogenously generated NO detection study, clear intracellular red fluorescence was observed in the presence of S-nitroso-N-acetyl-D,L-penicillamine (SNAP, a kind of NO releasing agent). In endogenously generated NO detection study, increasing incubation time of RH with lipopolysaccharied (LPS) pre-treated cells could obtain a highly fluorescent cell image. These cell imaging results demonstrated that RH can efficiently penetrate into Raw 264.7 cells and be used for detection of exogenously and endogenously generated nitric oxide. (C) 2011 Elsevier B.V. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.aca.2011.10.005 http://hdl.handle.net/11536/14901 |
ISSN: | 0003-2670 |
DOI: | 10.1016/j.aca.2011.10.005 |
期刊: | ANALYTICA CHIMICA ACTA |
Volume: | 708 |
Issue: | 1-2 |
起始頁: | 141 |
結束頁: | 148 |
顯示於類別: | 期刊論文 |