標題: Colorimetric and Fluorescent Dual-Mode Immunoassay Based on Plasmon-Enhanced Fluorescence of Polymer Dots for Detection of PSA in Whole Blood
作者: You, Pei-Yun
Li, Fang-Chu
Liu, Ming-Ho
Chan, Yang-Hsiang
應用化學系
Department of Applied Chemistry
關鍵字: semiconducting polymer dots;dual-mode test strip;biomarker detection;PSA POC monitoring;whole-blood analysis
公開日期: 13-Mar-2019
摘要: Although enormous efforts have been devoted to the development of new types of fluorometric immuno-chromatographic test strip (ICTS) with improved sensitivity over the past years, it still remains a big challenge to design ICTS with colorimetric and fluorescent bimodal signal readout for rapid yet accurate detection of cancer markers in a clinic. Scientists have tried to prepare bimodal reporters by combining fluorescent dyes with metal nanomaterials, but their fluorescence was easily quenched by metal nanomaterials through surface energy transfer, making dual colorimetric and fluorometric ICTS very difficult to be achieved. As compared to conventional fluorescent probes, semiconducting polymer dots (Pdots) exhibit extraordinary fluorescence brightness and facile surface functionalization, which are very suitable to be engineered as bimodal signal reporting reagents. Here, we integrated highly fluorescent Pdots with strongly plasmonic Au nanorods to form Pdot-Au hybrid nanocomposites with dual colorimetric and fluorescent readout abilities. We further utilized these nanohybrids in ICTS for qualitatively fast screening (colorimetry) as well as quantitatively accurate determination (fluorometry) of prostate-specific antigen (PSA) within 10 min. By taking advantage of the plasmon-enhanced fluorescence of Pdots on Au nanorods, this immunoassay possesses much better detection sensitivity of 1.07 pg/mL for PSA, which is at least 2 orders of magnitude lower than that of conventional fluorometric ICTS. Moreover, the direct detection of PSA from human whole blood collected without sample pretreatment makes this Pdot-based ICTS platform promising for on-site point-of-care diagnostics.
URI: http://dx.doi.org/10.1021/acsami.9b00204
http://hdl.handle.net/11536/149038
ISSN: 1944-8244
DOI: 10.1021/acsami.9b00204
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 11
起始頁: 9841
結束頁: 9849
Appears in Collections:Articles