标题: | In situ observation of cell-detachment process initiated by femtosecond laser-induced stress wave |
作者: | Maezawa, Yasuyo Hosokawa, Yoichiroh Okano, Kazunori Matsubara, Mie Masuhara, Hiroshi 应用化学系 应用化学系分子科学硕博班 Department of Applied Chemistry Institute of Molecular science |
公开日期: | 1-十月-2010 |
摘要: | When a stress wave generated by focusing a femtosecond laser is loaded on an animal cell adhered on a substrate, the cell is detached from the substrate. There are two possible mechanisms for the cell detachment: (a) The cell is detached from a scaffold coated on a glass plate, and (b) the cell is detached from the glass plate with the scaffold. In this work, we have studied the cell-detachment mechanism by visualizing the scaffold with a fluorescence probe of quantum dots. When the cell was detached from the substrate, fluorescence from the scaffold simultaneously disappeared from the glass plate, although the scaffold was not irradiated by the laser. This indicates that detachment due to the stress wave is attributed to mechanism (a). On the other hand, when the cell was detached from the substrate by a trypsin treatment, the fluorescence from the scaffold remained, suggesting mechanism (b). By comparing both results, it is considered that physiological damage of the cell membrane during the detachment process by femtosecond laser-induced stress wave is less than that due to the trypsin treatment. |
URI: | http://dx.doi.org/10.1007/s00339-010-5771-4 http://hdl.handle.net/11536/150089 |
ISSN: | 0947-8396 |
DOI: | 10.1007/s00339-010-5771-4 |
期刊: | APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING |
Volume: | 101 |
起始页: | 127 |
结束页: | 131 |
显示于类别: | Articles |