標題: | Sub-diffraction-limit imaging using mode multiplexing |
作者: | Wang, Nan He, Jinping Miyazaki, Jun Tsurui, Hiromichi Kobayashi, Takayoshi 電子物理學系 Department of Electrophysics |
關鍵字: | subtraction microscopy;multicolor imaging;quantum dot;mode multiplexing;fluorescence imaging |
公開日期: | 1-一月-2015 |
摘要: | Simultaneous two-color subtraction microscopy using mode multiplexing is realized experimentally. The samples are irradiated with single laser diode at wavelength of 445 nm. Then the beam split laser spots generate separate solid and donut spatial modes and are multiplexed with modulators for simultaneous excitation. The produced fluorescence signals are back collected and further divided into two color bands with dichroic mirrors. Then they are detected with two photomultipliers and demultiplexed in four lock-in amplifiers. Four fluorescence images are recorded in every scan and resolution enhanced images are obtained in two color channels after applying the subtraction strategy. With this method, imaging results of microspheres stained with organic dyes and mesenteric lymph nodes of a mouse labeled with quantum dots (Q525/650) are realized. Improvement of 20% similar to 30% in resolving power of the two color channels compared with confocal microscopy is achieved in with corresponding subtraction factor of about 0.3. |
URI: | http://dx.doi.org/10.1117/12.2186137 http://hdl.handle.net/11536/150648 |
ISBN: | 978-1-62841-846-0 |
ISSN: | 0277-786X |
DOI: | 10.1117/12.2186137 |
期刊: | SCANNING MICROSCOPIES 2015 |
Volume: | 9636 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 會議論文 |