標題: Sub-diffraction-limit imaging using mode multiplexing
作者: Wang, Nan
He, Jinping
Miyazaki, Jun
Tsurui, Hiromichi
Kobayashi, Takayoshi
電子物理學系
Department of Electrophysics
關鍵字: subtraction microscopy;multicolor imaging;quantum dot;mode multiplexing;fluorescence imaging
公開日期: 1-一月-2015
摘要: Simultaneous two-color subtraction microscopy using mode multiplexing is realized experimentally. The samples are irradiated with single laser diode at wavelength of 445 nm. Then the beam split laser spots generate separate solid and donut spatial modes and are multiplexed with modulators for simultaneous excitation. The produced fluorescence signals are back collected and further divided into two color bands with dichroic mirrors. Then they are detected with two photomultipliers and demultiplexed in four lock-in amplifiers. Four fluorescence images are recorded in every scan and resolution enhanced images are obtained in two color channels after applying the subtraction strategy. With this method, imaging results of microspheres stained with organic dyes and mesenteric lymph nodes of a mouse labeled with quantum dots (Q525/650) are realized. Improvement of 20% similar to 30% in resolving power of the two color channels compared with confocal microscopy is achieved in with corresponding subtraction factor of about 0.3.
URI: http://dx.doi.org/10.1117/12.2186137
http://hdl.handle.net/11536/150648
ISBN: 978-1-62841-846-0
ISSN: 0277-786X
DOI: 10.1117/12.2186137
期刊: SCANNING MICROSCOPIES 2015
Volume: 9636
起始頁: 0
結束頁: 0
顯示於類別:會議論文


文件中的檔案:

  1. dbe9b13b95de1865711fad9f230e5eb3.pdf

若為 zip 檔案,請下載檔案解壓縮後,用瀏覽器開啟資料夾中的 index.html 瀏覽全文。