Title: Surface Cleaning of the Nanowire Field-Effect Transistor for Gene Detection
Authors: Wu, Chi-Chang
Liu, Fu-Ken
Lin, Ling-Hung
Pang, See-Tong
Chuang, Cheng-Keng
Pan, Tung-Ming
Ou, Keng-Liang
Ko, Fu-Hsiang
材料科學與工程學系
Department of Materials Science and Engineering
Keywords: Nanowire;Biosensor;Surface Cleaning;DNA
Issue Date: 1-Dec-2011
Abstract: The efficiency of DNA immobilization by using various surface cleaning methods is studied in this work. The degree of surface cleaning is evaluated by the surface tension measurement to reveal the contribution from the polar and apolar terms. The observation from the fluorescent microscope images indicates the effectiveness of surface clean by the acetone and ethanol mixtures, as well as the sulphuric acid and hydrogen peroxide mixtures. We also fabricate a series of back-gated, 60-nm nanowired (NW) field-effect transistor (FET) sensors for mutation gene detection by following the developed acetone and ethanol mixtures. Electrical properties of the NWFET sensor demonstrate the n-channel depletion characteristics. The current of the sensor is reduced once the attachment of negative charge molecules. The single-stranded capture DNA is chemically immobilized onto the surface of silicon NWFET by three-step reactions. The sensor surface demonstrates the great performance of current shift after the suitable cleaning. The NWFET sensor is successfully applied to detect the BRAF(V599E) mutation genes from the hybridized processes. The sensing behaviour estimated from the electrical signal reaches the femtomolar level.
URI: http://dx.doi.org/10.1166/jnn.2011.3952
http://hdl.handle.net/11536/15087
ISSN: 1533-4880
DOI: 10.1166/jnn.2011.3952
Journal: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume: 11
Issue: 12
Begin Page: 10639
End Page: 10643
Appears in Collections:Conferences Paper