Simultaneous observation of cell behavior on multiple substrates with different heights

Abstract

We propose a microscopic system that can perform simultaneous time-lapse recordings for studying cell behavior on different substrates. The microscopic objective lens is controlled up and down so that the focal plane is matched with different heights of each surface. The captured video includes two types of images with a given time interval. Later, the focused image corresponding to each substrate is rearranged every time segment. To confirm the working principle, we show the experimental results for smooth muscle cells (SMCs) by using two different substrates.