標題: Detection of Multiple Embryo Growth Factors by Bead -Based Digital Microfluidic Chip in Embryo Culture Medium
作者: Lee, Chia-Tung
Tseng, Hsueh-Yang
Jiang, Yi-Ting
Haung, Cheng-Yeh
Lee, Meng-Shiue
Hsu, Wen-syang
交大名義發表
機械工程學系
National Chiao Tung University
Department of Mechanical Engineering
公開日期: 1-一月-2018
摘要: -In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL -to and TNF-a in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead -based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF-a calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL -1(3 calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min.
URI: http://hdl.handle.net/11536/152004
ISBN: 978-1-5386-5273-2
ISSN: 2474-3747
期刊: 2018 13TH ANNUAL IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS (NEMS 2018)
起始頁: 119
結束頁: 122
顯示於類別:會議論文