標題: | Detection of Multiple Embryo Growth Factors by Bead -Based Digital Microfluidic Chip in Embryo Culture Medium |
作者: | Lee, Chia-Tung Tseng, Hsueh-Yang Jiang, Yi-Ting Haung, Cheng-Yeh Lee, Meng-Shiue Hsu, Wen-syang 交大名義發表 機械工程學系 National Chiao Tung University Department of Mechanical Engineering |
公開日期: | 1-一月-2018 |
摘要: | -In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL -to and TNF-a in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead -based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF-a calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL -1(3 calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min. |
URI: | http://hdl.handle.net/11536/152004 |
ISBN: | 978-1-5386-5273-2 |
ISSN: | 2474-3747 |
期刊: | 2018 13TH ANNUAL IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS (NEMS 2018) |
起始頁: | 119 |
結束頁: | 122 |
顯示於類別: | 會議論文 |