標題: | Differentiation between normal and cancer cells with autofluorescence lifetime microscopy and nanosecond pulsed electric field effects |
作者: | Ohta, Nobuhiro Awasthi, Kamlesh Hsu, Hsin-Yun Hsieh, Pei-Ying 交大名義發表 應用化學系 應用化學系分子科學碩博班 National Chiao Tung University Department of Applied Chemistry Institute of Molecular science |
關鍵字: | Fluorescence lifetime microscopy (FLIM);endogenous fluorophore;nicotinamide adenine dinucleotide;cancer and normal cells;nanosecond pulsed electric field effect |
公開日期: | 1-一月-2019 |
摘要: | Fluorescence lifetime images of the endogenous nicotinamide adenine dinucleotide (NADH), which is well known as autofluorescence chromophores, were obtained for rat normal fibroblast cells (WFB) and H-ras oncogene-transfected WFB cancer cells (W31) and for human normal lung fibroblast (MRC-5) and human lung large carcinoma (HCI-H661). In both cases, the average lifetime of the NADH autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells might alter the level of coenzymes such as NADH. It is also shown that application of nanosecond pulsed electric field (nsPEF) induces apoptosis in these cell, resulting in the morphological changes and lengthening the autofluorescence lifetime of NADH. Furthermore, we found that nsPEF more efficiently affected cancer cells than normal cells in cell viability, suggesting the possibility of drug-free cancer therapy by nsPEF. |
URI: | http://dx.doi.org/10.1117/12.2509164 http://hdl.handle.net/11536/152014 |
ISBN: | 978-1-5106-2423-8 |
ISSN: | 0277-786X |
DOI: | 10.1117/12.2509164 |
期刊: | LABEL-FREE BIOMEDICAL IMAGING AND SENSING (LBIS) 2019 |
Volume: | 10890 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 會議論文 |