標題: | Hepatic expression of MxA and OAS1 in an ex vivo liver slice assay independently predicts treatment outcomes in chronic hepatitis C |
作者: | Cheng, J. -C. Yeh, Y. -J. Huang, Y. -H. Liang, K. -H. Chang, M. -L. Lin, C. -Y. Yeh, C. -T. 生物資訊及系統生物研究所 Institude of Bioinformatics and Systems Biology |
關鍵字: | End of therapy response;interferon-stimulated gene;sustained virological response |
公開日期: | 1-二月-2012 |
摘要: | . Antiviral effect of interferon is mediated by the expression of interferon-stimulated genes (ISGs). However, because of the difficulty in obtaining paired liver biopsies before and after interferon treatment, the key ISGs expressed in human hepatocytes and responsible for interferon-based antiviral activities in chronic hepatitis C remain illusive. Prior to a standard course of peginterferon plus ribavirin therapy, 104 patients underwent a liver biopsy. A small piece of the liver biopsy sample from each patient was submitted for ex vivo tissue culture in the presence or absence of interferon. Hepatic expression of 8 ISGs was detected by RT-PCR. The ISG expression patterns and clinicopathological variables were correlated with subsequent treatment outcomes. Multivariate logistic regression analysis showed that hepatic MxA expression (P = 0.008) and leucocyte count (P = 0.040) independently predicted the end of therapeutic virological response, while hepatic OAS1 expression (P = 0.003), genotype 1 (P = 0.002), HCV-RNA level (P = 0.007), AST/ALT ratio (P = 0.004) and leucocyte count (P = 0.034) independently predicted the sustained virological response. Immunohistochemistry analysis showed that interferon-induced OAS1 expression localized to the hepatocytes. In conclusion, hepatic MxA and OAS1 expression predicted, respectively, the end of therapeutic and sustained virological responses in interferon-based treatment of chronic hepatitis C. |
URI: | http://dx.doi.org/10.1111/j.1365-2893.2011.01538.x http://hdl.handle.net/11536/15242 |
ISSN: | 1352-0504 |
DOI: | 10.1111/j.1365-2893.2011.01538.x |
期刊: | JOURNAL OF VIRAL HEPATITIS |
Volume: | 19 |
Issue: | 2 |
起始頁: | E154 |
結束頁: | E162 |
顯示於類別: | 期刊論文 |