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dc.contributor.authorTsai, Hsiou-Hsinen_US
dc.contributor.authorYang, Kai-Chiangen_US
dc.contributor.authorWu, Meng-Huangen_US
dc.contributor.authorChen, Jung-Chihen_US
dc.contributor.authorTseng, Ching-Lien_US
dc.date.accessioned2019-10-05T00:08:48Z-
dc.date.available2019-10-05T00:08:48Z-
dc.date.issued2019-08-02en_US
dc.identifier.issn1661-6596en_US
dc.identifier.urihttp://dx.doi.org/10.3390/ijms20164024en_US
dc.identifier.urihttp://hdl.handle.net/11536/152876-
dc.description.abstractThe culture environment plays an important role for stem cells' cultivation. Static or dynamic culture preserve differential potentials to affect human mesenchymal stem cells' (hMSCs) proliferation and differentiation. In this study, hMSCs were seeded on fiber disks and cultured in a bidirectional-flow bioreactor or spinner-flask bioreactor with a supplement of osteogenic medium. The hMSCs' proliferation, osteogenic differentiation, and extracellular matrix deposition of mineralization were demonstrated. The results showed that the spinner flask improved cell viability at the first two weeks while the bidirectional-flow reactor increased the cell proliferation of hMSCs through the four-week culture period. Despite the flow reactor having a higher cell number, a lower lactose/glucose ratio was noted, revealing that the bidirectional-flow bioreactor provides better oxygen accessibility to the cultured cells/disk construct. The changes of calcium ions in the medium, the depositions of Ca2+ in the cells/disk constructs, and alkaline phosphate/osteocalcin activities showed the static culture of hMSCs caused cells to mineralize faster than the other two bioreactors but without cell proliferation. Otherwise, cells were distributed uniformly with abundant extracellular matrix productions using the flow reactor. This reveals that the static and dynamic cultivations regulated the osteogenic process differently in hMSCs. The bidirectional-flow bioreactor can be used in the mass production and cultivation of hMSCs for applications in bone regenerative medicine.en_US
dc.language.isoen_USen_US
dc.subjectdynamic cultureen_US
dc.subjectflow reactoren_US
dc.subjectmesenchymal stem cellsen_US
dc.subjectproliferationen_US
dc.subjectdifferentiationen_US
dc.subjectosteogenesisen_US
dc.subjectbone regenerationen_US
dc.titleThe Effects of Different Dynamic Culture Systems on Cell Proliferation and Osteogenic Differentiation in Human Mesenchymal Stem Cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms20164024en_US
dc.identifier.journalINTERNATIONAL JOURNAL OF MOLECULAR SCIENCESen_US
dc.citation.volume20en_US
dc.citation.issue16en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000484411100181en_US
dc.citation.woscount0en_US
Appears in Collections:Articles