標題: Signal Enhancement of Silicon Nanowire Field-Effect Transistor Immunosensors by RNA Aptamer
作者: Vu, Cao-An
Hu, Wen-Pin
Yang, Yuh-Shyong
Chan, Hardy Wai-Hong
Chen, Wen-Yih
生物科技學系
Department of Biological Science and Technology
公開日期: 17-九月-2019
摘要: Silicon nanowire field-effect transistors (SiNW-FETs) have been demonstrated as a highly sensitive platform for label-free detection of a variety of biological and chemical entities. However, detecting signal from immunoassays by nanoFETs is severely hindered by the distribution of different charged groups of targeted entities, their binding orientation, and distances to the surface of the FET. Aptamers have been widely applied as a recognition element for plentiful biosensors because of small molecular sizes and moderate to high specific binding affinity with different types of molecules. In this study, we propose an effective approach to enhance the electrical responses of both direct (6x-histidine) and sandwich (amyloid beta 1-42) immunoassays in SiNW-FETs with R18, a highly negative charged RNA aptamer against rabbit immunoglobulin G (IgG). Empirical results presented that the immunosensors targeted with R18 expressed a significantly stabilized and amplified signal compared to the ones without this aptamer. The research outcome provides applicability of the highly negative charged aptamer as a bioamplifier for immunoassays by FETs.
URI: http://dx.doi.org/10.1021/acsomega.9b01264
http://hdl.handle.net/11536/153105
ISSN: 2470-1343
DOI: 10.1021/acsomega.9b01264
期刊: ACS OMEGA
Volume: 4
Issue: 12
起始頁: 14765
結束頁: 14771
顯示於類別:期刊論文