Full metadata record
DC FieldValueLanguage
dc.contributor.authorWu, Chia-Taen_US
dc.contributor.authorLin, Fei-Hungen_US
dc.contributor.authorLee, Yu-Tzuen_US
dc.contributor.authorKu, Min-Shoen_US
dc.contributor.authorLue, Ko-Haungen_US
dc.date.accessioned2020-02-02T23:54:38Z-
dc.date.available2020-02-02T23:54:38Z-
dc.date.issued2019-12-01en_US
dc.identifier.issn1684-1182en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.jmii.2019.03.002en_US
dc.identifier.urihttp://hdl.handle.net/11536/153590-
dc.description.abstractBackground: Asthma is a heterogeneous inflammatory disorder of the airway. A Th2 response usually contributes to high levels of allergen-specific IgE and eosinophilic airway inflammation. Several findings have demonstrated that neutrophils, not eosinophils, are the major inflammatory cells in chronic asthma patients with steroid-resistance. Lactobacillus rhammosus GG (LGG) exhibits anti-inflammatory properties on OVA-induced acute airway inflammation. Objective: We hypothesized that orally administrated LGG should reduce airway remodeling in chronic experimental models. Methods: Female Balb/c mice were sensitized with OVA. LGG was used to investigate whether oral administrations of LGG inhibited OVA-induced airway inflammation in a chronic asthma model and the different intervention times between LGG pre-treatment and post-treatment groups. BALF was analyzed with Liu's stain and ELISA assay. Lung histopathology was assayed with HE, IHC and Masson's trichrome staining. Lung tissues were assayed with PCR (T-bet, GATA3, RORrt and Foxp3). Many cytokines were detected in the serum and BALF. Results: LGG significantly decreased the number of infiltrating inflammatory cells. We also found that the oral LGG group suppressed not only Th2 cytokine, but also IL-17, TNF-alpha and HMGB1 in the BALF levels. However, GATA3 and RORrt decreased significantly in the RNA level in the LGG groups, but the T-bet and Foxp3 increased in the RNA level. Conclusions: LGG not only had anti-inflammatory effects on OVA-induced airway inflammation, but also improved airway remodeling and collagen expression in the chronic asthma mouse model. Moreover, LGG might be an additional or supplementary therapy for allergic airway diseases. Copyright (C) 2019, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.en_US
dc.language.isoen_USen_US
dc.subjectAsthmaen_US
dc.subjectAirway remodelingen_US
dc.subjectLGGen_US
dc.subjectRORrten_US
dc.subjectFoxp3en_US
dc.titleEffect of Lactobacillus rhamnosus GG immunopathologic changes in chronic mouse asthma modelen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jmii.2019.03.002en_US
dc.identifier.journalJOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTIONen_US
dc.citation.volume52en_US
dc.citation.issue6en_US
dc.citation.spage911en_US
dc.citation.epage919en_US
dc.contributor.department生物科技學院zh_TW
dc.contributor.departmentCollege of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000502816900010en_US
dc.citation.woscount0en_US
Appears in Collections:Articles