標題: | Microanalysis Using Acoustically Actuated Droplets Pinned Onto a Thread |
作者: | Yang, Tzu-Hsien Yang, Hao-Chun Chang, Cheng-Hao Prabhu, Gurpur Rakesh D. Urban, Pawel L. 應用化學系 Department of Applied Chemistry |
關鍵字: | Acoustic levitation;automation;enzyme assay;microdroplet assay;open-source electronics |
公開日期: | 1-一月-2019 |
摘要: | An acoustic wave-assisted microscale assay platform is demonstrated. Liquid droplets containing samples and reagents are dispensed and pinned onto a hydrophobic thread, and actuated by sound waves. The transport of droplets is achieved by modulating sound emitted by two sources. The resulting sound intensity gradient pushes the droplets in one direction. The available distance of droplet actuation is related to droplet size. The moving droplets merge at the end of their actuation range, and the merged droplet travels further due to its greater size. Fluorometric analysis of the merged droplet is accomplished by positioning an excitation light source above a defined interrogation zone along the thread. The emitted light is analyzed by a miniature spectrometer positioned orthogonally to the excitation light beam. The sound sources and detection system are controlled by universal electronic modules (Raspberry Pi, Arduino). The system is operated automatically with the aid of a custom-developed graphical user interface. Here, we demonstrate its application in enzyme assays (trypsin, beta-galactosidase). In-droplet enzymatic reaction progress curves are readily obtained. The actuated droplets can be put in motion and stopped without a significant delay. Only microliter-range volumes of sample and reagent are consumed in every analysis, while sub-milliliter volumes of chemical waste are produced. |
URI: | http://dx.doi.org/10.1109/ACCESS.2019.2948642 http://hdl.handle.net/11536/153795 |
ISSN: | 2169-3536 |
DOI: | 10.1109/ACCESS.2019.2948642 |
期刊: | IEEE ACCESS |
Volume: | 7 |
起始頁: | 154743 |
結束頁: | 154749 |
顯示於類別: | 期刊論文 |