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dc.contributor.authorLin, Chia-Hungen_US
dc.contributor.authorLin, Ming-Jieen_US
dc.contributor.authorHuang, Jie-Deen_US
dc.contributor.authorChuang, Yu-Shengen_US
dc.contributor.authorKuo, Yu-Fenen_US
dc.contributor.authorChen, Jung-Chihen_US
dc.contributor.authorWu, Ching-Chouen_US
dc.date.accessioned2020-05-05T00:02:25Z-
dc.date.available2020-05-05T00:02:25Z-
dc.date.issued2020-02-01en_US
dc.identifier.urihttp://dx.doi.org/10.3390/s20030771en_US
dc.identifier.urihttp://hdl.handle.net/11536/154233-
dc.description.abstractThe sensing properties of immunosensors are determined not only by the amount of immobilized antibodies but also by the number of effective antigen-binding sites of the immobilized antibody. Protein A (PA) exhibits a high degree of affinity with the Fc part of IgG antibody to feasibly produce oriented antibody immobilization. This work proposes a simple method to control the PA surface density on gold nanostructure (AuNS)-deposited screen-printed carbon electrodes (SPCEs) by mixing concentration-varied PA and bovine serum albumin (BSA), and to explore the effect of PA density on the affinity attachment of anti-salbutamol (SAL) antibodies by electrochemical impedance spectroscopy. A concentration of 100 mu g/mL PA and 100 mu g/mL BSA can obtain a saturated coverage on the 3-mercaptoproponic acid (MPA)/AuNS/SPCEs and exhibit a 50% PA density to adsorb the amount of anti-SAL, more than other concentration-varied PA/BSA-modified electrodes. Compared with the randomly immobilized anti-SAL/MPA/AuNS/SPCEs and the anti-SAL/PA(100 mu g/mL):BSA(0 mu g/mL)/MPA/AuNS/SPCE, the anti-SAL/PA(100 mu g/mL): BSA(100 mu g/mL)/MPA/AuNS/SPCE-based immunosensors have better sensing properties for SAL detection, with an extremely low detection limit of 0.2 fg/mL and high reproducibility (<2.5% relative standard deviation). The mixture of PA(100 mu g/mL):BSA(100 mu g/mL) for the modification of AuNS/SPCEs has great promise for forming an optimal protein layer for the oriented adsorption of IgG antibodies to construct ultrasensitive SAL immunosensors.en_US
dc.language.isoen_USen_US
dc.subjectprotein Aen_US
dc.subjectsurface densityen_US
dc.subjectoriented immobilizationen_US
dc.subjectelectrochemical impedance spectroscopyen_US
dc.subjectimmunosensoren_US
dc.titleLabel-Free Impedimetric Immunosensors Modulated by Protein A/Bovine Serum Albumin Layer for Ultrasensitive Detection of Salbutamolen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/s20030771en_US
dc.identifier.journalSENSORSen_US
dc.citation.volume20en_US
dc.citation.issue3en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000517786200195en_US
dc.citation.woscount0en_US
Appears in Collections:Articles