標題: Artificial peptide-controlled protein release of Zn2+-triggered, self-assembled histidine-tagged protein microparticle
作者: Chen, Tzu-Yin
Cheng, Wan-Jung
Horng, Jia-Cherng
Hsu, Hsin-Yun
交大名義發表
應用化學系
應用化學系分子科學碩博班
National Chiao Tung University
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Protein microparticle;Metal-Induced self-assembly;His-tag;Collagen-Mimetic;Peptide
公開日期: 1-Mar-2020
摘要: Protein microparticles have received attention as drug delivery systems because of their high protein stability and prolonged release in vivo. However, most current preparation processes introduce chemical crosslinkers, which often lead to protein inactivation and limit drug efficacy in delivery systems. In this study, we employed the well-known hexahistidine (His)-tag recombinant protein technology and a metal-triggerable collagen-mimetic peptide to enhance the binding strength between the protein and metal ion and fine-tuned the protein drug release. His-tagged proteins self-assembled to form microparticles (similar to 2 mu m) upon zinc ion treatment and sustained protein drug release was achieved in physiological saline. The results also indicated that by adjusting the peptide concentration and N- and C-terminal hexahistidine-tags, protein release could be controlled. Moreover, no protein denaturation was observed. We developed a universal strategy for facile protein microparticle fabrication under mild conditions and we demonstrated its potential as a therapeutics formulation with tunable protein release.
URI: http://dx.doi.org/10.1016/j.colsurfb.2019.110644
http://hdl.handle.net/11536/154403
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2019.110644
期刊: COLLOIDS AND SURFACES B-BIOINTERFACES
Volume: 187
起始頁: 0
結束頁: 0
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