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dc.contributor.authorChang, Po-Hanen_US
dc.contributor.authorWeng, Chang-Chingen_US
dc.contributor.authorLi, Bor-Ranen_US
dc.contributor.authorLi, Yaw-Kuenen_US
dc.date.accessioned2020-07-01T05:22:06Z-
dc.date.available2020-07-01T05:22:06Z-
dc.date.issued2020-03-01en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2019.111969en_US
dc.identifier.urihttp://hdl.handle.net/11536/154520-
dc.description.abstractWe report a peptide-based sensor that involves a multivalent interaction with L-ascorbate 6-phosphate lactonase (UlaG), a protein marker of Streptococcus pneumonia. By integrating the antifouling feature of the sensor, we significantly improved the signal-to-noise ratio of UlaG detection. The antifouling surface was fabricated via electrodeposition using an equivalent mixture of 4-amino-N,N,N-trimethylanilinium and 4-aminobenzenesulfonate. This antifouling layer not only effectively reduces the non-specific adsorption on the biosensor but also decreases the charge transfer resistance (R-et) of the screen-printed carbon electrode. The aniline-modified 57 peptide, an UlaG-binding peptide, was pre-synthesized and further electrochemically modified to bind onto the antifouling layer. Bio-electrochemical analysis confirms that the antifouling 57-peptide sensor binds strongly to the UlaG with a dissociation constant (K-d) = 0.5 nM. This strong interaction can be attributed to a multivalent interaction between the biosensor and the heximeric form of UlaG. To demonstrate the potential for clinical application, further detection of Streptococcus pneumonia from 50 to 5 x 10(4) CFU/mL were successfully performed in 25% human serum.en_US
dc.language.isoen_USen_US
dc.subjectAntifoulingen_US
dc.subjectElectrodepositionen_US
dc.subjectL-ascorbate-6-phosphate lactonaseen_US
dc.subjectMultivalent interactionen_US
dc.subjectPeptide-based biosensoren_US
dc.titleAn antifouling peptide-based biosensor for determination of Streptococcus pneumonia markers in human serumen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bios.2019.111969en_US
dc.identifier.journalBIOSENSORS & BIOELECTRONICSen_US
dc.citation.volume151en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department交大名義發表zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentNational Chiao Tung Universityen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000538862900017en_US
dc.citation.woscount0en_US
Appears in Collections:Articles