完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chang, Po-Han | en_US |
dc.contributor.author | Weng, Chang-Ching | en_US |
dc.contributor.author | Li, Bor-Ran | en_US |
dc.contributor.author | Li, Yaw-Kuen | en_US |
dc.date.accessioned | 2020-07-01T05:22:06Z | - |
dc.date.available | 2020-07-01T05:22:06Z | - |
dc.date.issued | 2020-03-01 | en_US |
dc.identifier.issn | 0956-5663 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.bios.2019.111969 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/154520 | - |
dc.description.abstract | We report a peptide-based sensor that involves a multivalent interaction with L-ascorbate 6-phosphate lactonase (UlaG), a protein marker of Streptococcus pneumonia. By integrating the antifouling feature of the sensor, we significantly improved the signal-to-noise ratio of UlaG detection. The antifouling surface was fabricated via electrodeposition using an equivalent mixture of 4-amino-N,N,N-trimethylanilinium and 4-aminobenzenesulfonate. This antifouling layer not only effectively reduces the non-specific adsorption on the biosensor but also decreases the charge transfer resistance (R-et) of the screen-printed carbon electrode. The aniline-modified 57 peptide, an UlaG-binding peptide, was pre-synthesized and further electrochemically modified to bind onto the antifouling layer. Bio-electrochemical analysis confirms that the antifouling 57-peptide sensor binds strongly to the UlaG with a dissociation constant (K-d) = 0.5 nM. This strong interaction can be attributed to a multivalent interaction between the biosensor and the heximeric form of UlaG. To demonstrate the potential for clinical application, further detection of Streptococcus pneumonia from 50 to 5 x 10(4) CFU/mL were successfully performed in 25% human serum. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Antifouling | en_US |
dc.subject | Electrodeposition | en_US |
dc.subject | L-ascorbate-6-phosphate lactonase | en_US |
dc.subject | Multivalent interaction | en_US |
dc.subject | Peptide-based biosensor | en_US |
dc.title | An antifouling peptide-based biosensor for determination of Streptococcus pneumonia markers in human serum | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.bios.2019.111969 | en_US |
dc.identifier.journal | BIOSENSORS & BIOELECTRONICS | en_US |
dc.citation.volume | 151 | en_US |
dc.citation.spage | 0 | en_US |
dc.citation.epage | 0 | en_US |
dc.contributor.department | 交大名義發表 | zh_TW |
dc.contributor.department | 分子醫學與生物工程研究所 | zh_TW |
dc.contributor.department | 應用化學系 | zh_TW |
dc.contributor.department | National Chiao Tung University | en_US |
dc.contributor.department | Institute of Molecular Medicine and Bioengineering | en_US |
dc.contributor.department | Department of Applied Chemistry | en_US |
dc.identifier.wosnumber | WOS:000538862900017 | en_US |
dc.citation.woscount | 0 | en_US |
顯示於類別: | 期刊論文 |