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dc.contributor.authorHuang, Yung-Anen_US
dc.contributor.authorHsu, Chih-Hsuanen_US
dc.contributor.authorChiu, Ho-Chiehen_US
dc.contributor.authorHsi, Pei-Yuen_US
dc.contributor.authorHo, Chris T.en_US
dc.contributor.authorLo, Wei-Lunen_US
dc.contributor.authorHwang, Ericen_US
dc.date.accessioned2020-07-01T05:22:08Z-
dc.date.available2020-07-01T05:22:08Z-
dc.date.issued2020-05-01en_US
dc.identifier.issn0021-9533en_US
dc.identifier.urihttp://dx.doi.org/10.1242/jcs.241992en_US
dc.identifier.urihttp://hdl.handle.net/11536/154551-
dc.description.abstractMicrotubules (MTs) are the most abundant cytoskeleton in neurons, and control multiple facets of their development. While the MT-organizing center (MTOC) in mitotic cells is typically located at the centrosome, the MTOC in neurons switches to non-centrosomal sites. A handful of cellular components have been shown to promote non-centrosomal MT (ncMT) formation in neurons, yet the regulation mechanism remains unknown. Here, we demonstrate that the small GTPase Ran is a key regulator of ncMTs in neurons. Using an optogenetic tool that enables light-induced local production of RanGTP, we demonstrate that RanGTP promotes ncMT plus-end growth along the neurite. Additionally, we discovered that actin waves drive the anterograde transport of RanGTP. Pharmacological disruption of actin waves abolishes the enrichment of RanGTP and reduces growing ncMT plus-ends at the neurite tip. These observations identify a novel regulation mechanism for ncMTs and pinpoint an indirect connection between the actin and MT cytoskeletons in neurons.en_US
dc.language.isoen_USen_US
dc.subjectMicrotubulesen_US
dc.subjectGrowth cone-like wavesen_US
dc.subjectPhotoactivationen_US
dc.subjectNeuronal developmenten_US
dc.subject+TIPsen_US
dc.titleActin waves transport RanGTP to the neurite tip to regulate non-centrosomal microtubules in neuronsen_US
dc.typeArticleen_US
dc.identifier.doi10.1242/jcs.241992en_US
dc.identifier.journalJOURNAL OF CELL SCIENCEen_US
dc.citation.volume133en_US
dc.citation.issue9en_US
dc.citation.spage0en_US
dc.citation.epage0en_US
dc.contributor.department交大名義發表zh_TW
dc.contributor.department生物科技學系zh_TW
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentNational Chiao Tung Universityen_US
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000534390800015en_US
dc.citation.woscount0en_US
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