Full metadata record
DC FieldValueLanguage
dc.contributor.authorLouis, Borisen_US
dc.contributor.authorCamacho, Rafaelen_US
dc.contributor.authorBresoli-Obach, Rogeren_US
dc.contributor.authorAbakumov, Sergeyen_US
dc.contributor.authorVandaele, Johannesen_US
dc.contributor.authorKudo, Testsuhiroen_US
dc.contributor.authorMasuhara, Hiroshien_US
dc.contributor.authorScheblykin, Ivan G.en_US
dc.contributor.authorHofkens, Johanen_US
dc.contributor.authorRocha, Susanaen_US
dc.date.accessioned2020-10-05T02:01:58Z-
dc.date.available2020-10-05T02:01:58Z-
dc.date.issued2020-09-14en_US
dc.identifier.issn1094-4087en_US
dc.identifier.urihttp://dx.doi.org/10.1364/OE.401557en_US
dc.identifier.urihttp://hdl.handle.net/11536/155389-
dc.description.abstractMultifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 mu m(3). The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 mu m fluorescent beads into an optical trap. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreementen_US
dc.language.isoen_USen_US
dc.titleFast-tracking of single emitters in large volumes with nanometer precisionen_US
dc.typeArticleen_US
dc.identifier.doi10.1364/OE.401557en_US
dc.identifier.journalOPTICS EXPRESSen_US
dc.citation.volume28en_US
dc.citation.issue19en_US
dc.citation.spage28656en_US
dc.citation.epage28671en_US
dc.contributor.department交大名義發表zh_TW
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentNational Chiao Tung Universityen_US
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000569207700115en_US
dc.citation.woscount0en_US
Appears in Collections:Articles