標題: Fast-tracking of single emitters in large volumes with nanometer precision
作者: Louis, Boris
Camacho, Rafael
Bresoli-Obach, Roger
Abakumov, Sergey
Vandaele, Johannes
Kudo, Testsuhiro
Masuhara, Hiroshi
Scheblykin, Ivan G.
Hofkens, Johan
Rocha, Susana
交大名義發表
應用化學系
National Chiao Tung University
Department of Applied Chemistry
公開日期: 14-九月-2020
摘要: Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 mu m(3). The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 mu m fluorescent beads into an optical trap. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
URI: http://dx.doi.org/10.1364/OE.401557
http://hdl.handle.net/11536/155389
ISSN: 1094-4087
DOI: 10.1364/OE.401557
期刊: OPTICS EXPRESS
Volume: 28
Issue: 19
起始頁: 28656
結束頁: 28671
顯示於類別:期刊論文