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dc.contributor.authorChiu, Yi-Rongen_US
dc.contributor.authorHo, Wei-Jenen_US
dc.contributor.authorChao, Jiun-Shuanen_US
dc.contributor.authorYuan, Chiun-Jyeen_US
dc.date.accessioned2014-12-08T15:22:47Z-
dc.date.available2014-12-08T15:22:47Z-
dc.date.issued2012-04-01en_US
dc.identifier.issn1388-0764en_US
dc.identifier.urihttp://dx.doi.org/829en_US
dc.identifier.urihttp://hdl.handle.net/11536/16096-
dc.description.abstractA novel horseradish peroxidase-encapsulated silica nanoparticle (SNP) was generated in this study under relatively mild conditions. The generated enzyme-encapsulated SNP were relatively uniform in size (average 70 +/- 14.3 nm), monodispersed, and spherical, as characterized by transmission electron microscopy and scanning electron microscopy. The horseradish peroxidase encapsulated in silica nanoparticle exhibits biological properties, such as a pH-dependent activity profile and k(m) value, similar to that of free enzymes. Furthermore, enzyme-encapsulated SNP exhibited good operational stability for the repetitive usage with a relative standard deviation of 5.1 % ( n = 10) and a high stability for long term storage (>60 days) at 4 degrees C. The feasibility of using enzyme-encapsulated SNP in prodrug cancer therapy was also demonstrated by its capability to convert the prodrug indole-3-acetic acid into cytotoxic peroxyl radicals and trigger the death of tumor cells. These results indicate that the developed enzyme-encapsulated SNP has potential in the applications of prodrug cancer therapy.en_US
dc.language.isoen_USen_US
dc.subjectEnzyme encapsulationen_US
dc.subjectSilicate nanoparticleen_US
dc.subjectHRPen_US
dc.subjectIAAen_US
dc.subjectProdrug cancer therapyen_US
dc.subjectNanomedicineen_US
dc.titleEnzyme-encapsulated silica nanoparticle for cancer chemotherapyen_US
dc.typeArticleen_US
dc.identifier.doi829en_US
dc.identifier.journalJOURNAL OF NANOPARTICLE RESEARCHen_US
dc.citation.volume14en_US
dc.citation.issue4en_US
dc.citation.epageen_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000302641000067-
dc.citation.woscount4-
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