完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Shiue, Tsun-Wei | en_US |
dc.contributor.author | Chen, Yen-Hao | en_US |
dc.contributor.author | Wu, Chi-Ming | en_US |
dc.contributor.author | Singh, Gyan | en_US |
dc.contributor.author | Chen, Hsing-Yin | en_US |
dc.contributor.author | Hung, Chen-Hsiung | en_US |
dc.contributor.author | Liaw, Wen-Feng | en_US |
dc.contributor.author | Wang, Yun-Ming | en_US |
dc.date.accessioned | 2014-12-08T15:23:14Z | - |
dc.date.available | 2014-12-08T15:23:14Z | - |
dc.date.issued | 2012-05-07 | en_US |
dc.identifier.issn | 0020-1669 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/16311 | - |
dc.description.abstract | The stable, water-soluble, and nonfluorescent FA-OMe can sense nitric oxide (NO) and form the intensely fluorescent product dA-FA-OMe via reductive deamination of the aromatic primary amine. The reaction is accompanied by a notable increase of the fluorescent quantum yield from 1.5 to 88.8%. The deamination mechanism of FA-OMe with NO was proposed in this study. The turn-on fluorescence signals were performed by suppression of photoinduced electron transfer (PeT), which was demonstrated by density functional theory (DFT) calculations of the components forming FA-OMe and dA-FA-OMe. Furthermore, FA-OMe showed water solubility and good stability at physiological pHs. Moreover, the selectivity study indicated that FA-OMe had high specificity for NO over other reactive oxygen/nitrogen species. In an endogenously generated NO detection study, increasing the incubation time of FA-OMe with lipopolysaccharide (LPS) pretreated Raw 264.7 murine macrophages could cause an enhanced fluorescence intensity image. In addition, a diffusion/localization cell imaging study showed that FA-OMe could be trapped in Raw 264.7 cells. These cell imaging results demonstrated that FA-OMe could be used as a turn-on fluorescent sensor for the detection of endogenously generated NO. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Nitric Oxide Turn-on Fluorescent Probe Based on Deamination of Aromatic Primary Monoamines | en_US |
dc.type | Article | en_US |
dc.identifier.journal | INORGANIC CHEMISTRY | en_US |
dc.citation.volume | 51 | en_US |
dc.citation.issue | 9 | en_US |
dc.citation.epage | 5400 | en_US |
dc.contributor.department | 分子醫學與生物工程研究所 | zh_TW |
dc.contributor.department | Institute of Molecular Medicine and Bioengineering | en_US |
dc.identifier.wosnumber | WOS:000303952900065 | - |
dc.citation.woscount | 20 | - |
顯示於類別: | 期刊論文 |