標題: | Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation) |
作者: | Segawa, Hiroki Okuno, Masanari Kano, Hideaki Leproux, Philippe Couderc, Vincent Hamaguchi, Hiro-o 應用化學系 應用化學系分子科學碩博班 Department of Applied Chemistry Institute of Molecular science |
公開日期: | 23-四月-2012 |
摘要: | We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), THG (third harmonic generation) and multiplex TSFG (third-order sum frequency generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall. (C) 2012 Optical Society of America |
URI: | http://dx.doi.org/10.1364/OE.20.009551 http://hdl.handle.net/11536/16343 |
ISSN: | 1094-4087 |
DOI: | 10.1364/OE.20.009551 |
期刊: | OPTICS EXPRESS |
Volume: | 20 |
Issue: | 9 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 期刊論文 |