標題: Label-free Detection of Prostate Specific Antigen Using a Silicon Nanobelt Field-effect Transistor
作者: Wu, Chi-Chang
Pan, Tung-Ming
Wu, Chung-Shu
Yen, Li-Chen
Chuang, Cheng-Keng
Pang, See-Tong
Yang, Yuh-Shyong
Ko, Fu-Hsiang
材料科學與工程學系
電子物理學系
Department of Materials Science and Engineering
Department of Electrophysics
關鍵字: Nanobelt;Field-effect Transistor;Biosensor;Prostate Specific Antigen;Label-free Detection
公開日期: 1-May-2012
摘要: In this study, we proposed a silicon nanobelt field-effect transistor (FET) to detect prostate specific antigen (PSA). The nanobelt FET device displayed n-channel depletion characteristics. The immobilization of prostate specific antibody (anti-PSA) molecules was attached onto the nanobelt FET surface by using the aldehyde groups of glutaraldehyde linked to the amino groups of 3-aminopropyltriethoxysilane (APTES). The shift in the drain current vs time curves of a nanobelt FET biosensor revealed that the electrical signal had a logarithmic relationship with respect to the concentration of the PSA, and detection capability was estimated in the 5 pg/mL level. To enhance the sensitivity of a nanobelt FET biosensor, this biosensor was designed by inserting arginine molecules between glutaraldehyde and APTES. Therefore, the detection capability of the developed sensor was extended to 50 fg/mL. Also, the relationship between the current shift and the logarithm of PSA concentration was exhibited linearity in the range 50 fg/mL - 500 pg/mL. The excellent electrical results of this label-free PSA nanobelt FET biosensor suggested that such biosensor might be potentially useful tools for biological research and future prostate cancer screening.
URI: http://hdl.handle.net/11536/16541
ISSN: 1452-3981
期刊: INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Volume: 7
Issue: 5
結束頁: 4432
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