標題: | Capulet and Slingshot share overlapping functions during Drosophila eye morphogenesis |
作者: | Lin, Chiao-Ming Lin, Pei-Yi Li, Yu-Chiao Hsu, Jui-Chou 生物科技學系 Department of Biological Science and Technology |
關鍵字: | Capulet;Slingshot;Twinstar;F-actin;Eye development |
公開日期: | 1-一月-1970 |
摘要: | "Background: CAP/Capulet (Capt), Slingshot (Ssh) and Cofilin/Twinstar (Tsr) are actin-binding proteins that restrict actin polymerization. Previously, it was shown that low resolution analyses of loss-of-function mutations in capt, ssh and tsr all show ectopic F-actin accumulation in various Drosophila tissues. In contrast, RNAi depletion of capt, tsr and ssh in Drosophila S2 cells all affect actin-based lamella formation differently. Whether loss of these three related genes might cause the same effect in the same tissue remains unclear. Methods: Loss-of-function mutant clones were generated using the MARCM or EGUF system whereas overexpression clones were generated using the Flip-out system. Immunostaining were then performed in eye imaginal discs with clones. FRAP was performed in cultured eye discs. Results: Here, we compared their loss-of-function phenotype at single-cell resolution, using a sheet of epithelial cells in the Drosophila eye imaginal disc as a model system. Surprisingly, we found that capt and ssh, but not tsr, mutant cells within and posterior to the morphogenetic furrow (MF) shared similar phenotypes. The capt/ssh mutant cells possessed: (1) hexagonal cell packing with discontinuous adherens junctions; and (2) largely complementary accumulation of excessive phosphorylated myosin light chain (p-MLC) and F-actin rings at the apical cortex. We further showed that the capt/ssh mutant phenotypes depended on the inactivation of protein kinase A (PKA) and activation of Rho. Conclusions: Although Capt, Ssh and Tsr were reported to negatively regulate actin polymerization, we found that Capt and Ssh, but not Tsr, share overlapping functions during eye morphogenesis." |
URI: | http://dx.doi.org/46 http://hdl.handle.net/11536/16667 |
ISSN: | 1021-7770 |
DOI: | 46 |
期刊: | JOURNAL OF BIOMEDICAL SCIENCE |
Volume: | 19 |
Issue: | |
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顯示於類別: | 期刊論文 |