标题: | Identifying the Regulative Role of NF-kappa B Binding Sites Within Promoter Region of Human Matrix Metalloproteinase 9 (mmp-9) by TNF-alpha Induction |
作者: | Wu, Hsi Tien Sie, Syu Sheng Kuan, Tang Ching Lin, Chih Sheng 生物科技学系 Department of Biological Science and Technology |
关键字: | Matrix metalloproteinases 9 (MMP-9);Nuclear factor-kappa B (NF-kappa B);Tumor necrosis factor-alpha (TNF-alpha);Transcription factor;Cell line H9c2 |
公开日期: | 1-一月-2013 |
摘要: | Matrix metalloproteinase 9 (MMP-9), a member of MMP family, is involved in many physiological processes, including cardiovascular disease (CVD). Tumor necrosis factor-alpha (TNF-alpha) is considered a cytokine with pleiotropic biological capabilities and leads to the process of CVD when TNF-alpha is abnormally released and stimulates MMP-9 expression and activation. In this study, we investigated the molecular mechanism of TNF-alpha-regulated MMP-9 expression. The experimental results confirm that TNF-alpha could upregulate MMP-9 expression in heart myoblast H9c2 cells of rat. To evaluate the MMP-9 regulation at transcriptional level, a DNA fragment of 2.2 kb (-2168/+18) of human mmp-9 promoter region was cloned and constructed in a vector of luciferase reporter gene. The 2.2-kb sequences were identified as having three candidate nuclear factor-kappa B (NF-kappa B) binding sites: NF-kappa B I (-1418/-1409), NF-kappa B II (-626/-617), and NF-kappa B III (-353/-345). A series of reporter vectors with the mutated NF-kappa B sites of mmp-9 promoter sequences were constructed and transfected into H9c2 cells. The results show that the NF-kappa B II binding site (-626/-617) within the promoter region of mmp-9 plays a key role in upregulation of mmp-9 expression by TNF-alpha induction. In addition, we also first identified that the NF-kappa B I, similar to c-Rel, might be one of the NF-kappa B families to regulate mmp-9 expression. |
URI: | http://dx.doi.org/10.1007/s12010-012-9958-3 http://hdl.handle.net/11536/21070 |
ISSN: | 0273-2289 |
DOI: | 10.1007/s12010-012-9958-3 |
期刊: | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY |
Volume: | 169 |
Issue: | 2 |
起始页: | 438 |
结束页: | 449 |
显示于类别: | Articles |
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