Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lu, Huai-En | en_US |
dc.contributor.author | Tsai, Ming-Song | en_US |
dc.contributor.author | Yang, Yao-Chen | en_US |
dc.contributor.author | Yuan, Chen-Ching | en_US |
dc.contributor.author | Wang, Tzu-Hao | en_US |
dc.contributor.author | Lin, Xi-Zhang | en_US |
dc.contributor.author | Tseng, Ching-Ping | en_US |
dc.contributor.author | Hwang, Shiaw-Min | en_US |
dc.date.accessioned | 2014-12-08T15:29:31Z | - |
dc.date.available | 2014-12-08T15:29:31Z | - |
dc.date.issued | 2011-08-01 | en_US |
dc.identifier.issn | 0014-4827 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.yexcr.2011.05.017 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/21227 | - |
dc.description.abstract | Generation of induced pluripotent stem (iPS) cells from somatic cells has been successfully achieved by ectopic expression of four transcription factors, Oct4, Sox2, Klf4 and c-Myc, also known as the Yamanaka factors. In practice, initial iPS colonies are picked based on their embryonic stem (ES) cell-like morphology, but often may go on to fail subsequent assays, such as the alkaline phosphate (AP) assay. In this study, we co-expressed through lenti-viral delivery the Yamanaka factors in amniotic fluid-derived (AF) cells. ES-like colonies were picked onto a traditional feeder layer and a high percentage AF-iPS with partial to no AP activity was found. Interestingly, we obtained an overwhelming majority of fully stained AP positive (AP+) AF-iPS colonies when colonies were first seeded on a feeder-free culture system, and then transferred to a feeder layer for expansion. Furthermore, colonies with no AP activity were not detected. This screening step decreased the variation seen between morphology and AP assay. We observed the AF-iPS colonies grown on the feeder layer with 28%AP+ colonies, 45% AP partially positive (AP+/-) colonies and 27% AP negative (AP-) colonies, while colonies screened by the feeder-free system were 84%AP+ colonies, 16% AP+/- colonies and no AP- colonies. The feeder-free screened AP+ AF-iPS colonies were also positive for pluripotent markers, OCT4, SOX2, NANOG,TRA-1-60,TRA-1-81,SSEA-3 and SSEA-4 as well as having differentiation abilities into three germ layers in vitro and in vivo. In this study, we report a simplistic, one-step method for selection of AP+ AF-iPS cells via feeder-free screening. (C) 2011 Elsevier Inc. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Amniotic fluid | en_US |
dc.subject | Induced pluripotent stem cells | en_US |
dc.subject | Feeder free | en_US |
dc.subject | Alkaline phosphatase | en_US |
dc.title | Selection of alkaline phosphatase-positive induced pluripotent stem cells from human amniotic fluid-derived cells by feeder-free system | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.yexcr.2011.05.017 | en_US |
dc.identifier.journal | EXPERIMENTAL CELL RESEARCH | en_US |
dc.citation.volume | 317 | en_US |
dc.citation.issue | 13 | en_US |
dc.citation.spage | 1895 | en_US |
dc.citation.epage | 1903 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000292409300011 | - |
dc.citation.woscount | 7 | - |
Appears in Collections: | Articles |
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