標題: Photoluminescent Gold Nanoclusters as Sensing Probes for Uropathogenic Escherichia coli
作者: Chan, Po-Han
Ghosh, Bhaswati
Lai, Hong-Zheng
Peng, Hwei-Ling
Mong, Kwok Kong Tony
Chen, Yu-Chie
生物科技學系
應用化學系
Department of Biological Science and Technology
Department of Applied Chemistry
公開日期: 15-三月-2013
摘要: Glycan-bound nanoprobes have been demonstrated as suitable sensing probes for bacteria containing glycan binding sites. In this study, we demonstrated a facile approach for generating glycan-bound gold nanoclusters (AuNCs). The generated AuNCs were used as sensing probes for corresponding target bacteria. Mannose-capped AuNCs (AuNCs@Mann) were generated and used as the model sensors for target bacteria. A one-step synthesis approach was employed to generate AuNCs@Mann. In this approach, an aqueous solution of tetrachloroauric acid and mannoside that functionized with a thiol group (Mann-SH) was stirred at room temperature for 48 h. The mannoside functions as reducing and capping agent. The size of the generated AuNCs@Mann is 1.95 +/- 0.27 nm, whereas the AuNCs with red photoluminescence have a maximum emission wavelength of similar to 630 nm (lambda(excitation) = 375 nm). The synthesis of the AuNCs@Mann was accelerated by microwave heating, which enabled the synthesis of the AuNCs@Mann to complete within 1 h. The generated AuNCs@Mann are capable of selectively binding to the urinary tract infection isolate Escherichia coli J96 containing the mannose binding protein FimH expressed on the type 1 pili. On the basis of the naked eye observation, the limit of detection of the sensing approach is as low as similar to 2 x 10(6) cells/mL.
URI: http://dx.doi.org/10.1371/journal.pone.0058064
http://hdl.handle.net/11536/21380
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0058064
期刊: PLOS ONE
Volume: 8
Issue: 3
結束頁: 
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