標題: | Photoluminescent Gold Nanoclusters as Sensing Probes for Uropathogenic Escherichia coli |
作者: | Chan, Po-Han Ghosh, Bhaswati Lai, Hong-Zheng Peng, Hwei-Ling Mong, Kwok Kong Tony Chen, Yu-Chie 生物科技學系 應用化學系 Department of Biological Science and Technology Department of Applied Chemistry |
公開日期: | 15-三月-2013 |
摘要: | Glycan-bound nanoprobes have been demonstrated as suitable sensing probes for bacteria containing glycan binding sites. In this study, we demonstrated a facile approach for generating glycan-bound gold nanoclusters (AuNCs). The generated AuNCs were used as sensing probes for corresponding target bacteria. Mannose-capped AuNCs (AuNCs@Mann) were generated and used as the model sensors for target bacteria. A one-step synthesis approach was employed to generate AuNCs@Mann. In this approach, an aqueous solution of tetrachloroauric acid and mannoside that functionized with a thiol group (Mann-SH) was stirred at room temperature for 48 h. The mannoside functions as reducing and capping agent. The size of the generated AuNCs@Mann is 1.95 +/- 0.27 nm, whereas the AuNCs with red photoluminescence have a maximum emission wavelength of similar to 630 nm (lambda(excitation) = 375 nm). The synthesis of the AuNCs@Mann was accelerated by microwave heating, which enabled the synthesis of the AuNCs@Mann to complete within 1 h. The generated AuNCs@Mann are capable of selectively binding to the urinary tract infection isolate Escherichia coli J96 containing the mannose binding protein FimH expressed on the type 1 pili. On the basis of the naked eye observation, the limit of detection of the sensing approach is as low as similar to 2 x 10(6) cells/mL. |
URI: | http://dx.doi.org/10.1371/journal.pone.0058064 http://hdl.handle.net/11536/21380 |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0058064 |
期刊: | PLOS ONE |
Volume: | 8 |
Issue: | 3 |
結束頁: | |
顯示於類別: | 期刊論文 |