Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping

doi:10.1021/ol303328t

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DC 欄位	值	語言
dc.contributor.author	Yeh, Hsu-Hua	en_US
dc.contributor.author	Chang, Shu-Lin	en_US
dc.contributor.author	Chiang, Yi-Ming	en_US
dc.contributor.author	Bruno, Kenneth S.	en_US
dc.contributor.author	Oakley, Berl R.	en_US
dc.contributor.author	Wu, Tung-Kung	en_US
dc.contributor.author	Wang, Clay C. C.	en_US
dc.date.accessioned	2014-12-08T15:29:54Z	-
dc.date.available	2014-12-08T15:29:54Z	-
dc.date.issued	2013-02-15	en_US
dc.identifier.issn	1523-7060	en_US
dc.identifier.uri	http://dx.doi.org/10.1021/ol303328t	en_US
dc.identifier.uri	http://hdl.handle.net/11536/21435	-
dc.description.abstract	We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA In A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA Delta R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.	en_US
dc.language.iso	en_US	en_US
dc.title	Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping	en_US
dc.type	Article	en_US
dc.identifier.doi	10.1021/ol303328t	en_US
dc.identifier.journal	ORGANIC LETTERS	en_US
dc.citation.volume	15	en_US
dc.citation.issue	4	en_US
dc.citation.spage	756	en_US
dc.citation.epage	759	en_US
dc.contributor.department	生物科技學系	zh_TW
dc.contributor.department	Department of Biological Science and Technology	en_US
dc.identifier.wosnumber	WOS:000315254500010	-
dc.citation.woscount	8	-
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