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dc.contributor.authorChen, Jian-Renen_US
dc.contributor.authorWu, Jia-Jiuanen_US
dc.contributor.authorLin, Justin Chun-Teen_US
dc.contributor.authorWang, Yuan-Chuenen_US
dc.contributor.authorYoung, Chiu-Chungen_US
dc.contributor.authorShieh, Chwen-Jenen_US
dc.contributor.authorHsu, Shih-Lanen_US
dc.contributor.authorChang, Cheih-Ming J.en_US
dc.date.accessioned2014-12-08T15:31:09Z-
dc.date.available2014-12-08T15:31:09Z-
dc.date.issued2013-07-19en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.chroma.2013.05.022en_US
dc.identifier.urihttp://hdl.handle.net/11536/22190-
dc.description.abstractIn this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-beta-carotenes from Dunaliella saline. Total concentration of 9-cis (134.7 mg/g) and trans-beta-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total beta-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total beta-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-beta-carotenes by low-density SAS was proved to be feasible and environmental benign. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.titleLow density supercritical fluids precipitation of 9-cis and all trans-beta-carotenes enriched particulates from Dunaliella salinaen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.chroma.2013.05.022en_US
dc.identifier.journalJOURNAL OF CHROMATOGRAPHY Aen_US
dc.citation.volume1299en_US
dc.citation.issueen_US
dc.citation.spage1en_US
dc.citation.epage9en_US
dc.contributor.department環境工程研究所zh_TW
dc.contributor.departmentInstitute of Environmental Engineeringen_US
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