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dc.contributor.authorHuang, Yung-Anen_US
dc.contributor.authorKao, Jun-Weien_US
dc.contributor.authorTseng, Dion Tzu-Huanen_US
dc.contributor.authorChen, Wen-Shinen_US
dc.contributor.authorChiang, Ming-Hanen_US
dc.contributor.authorHwang, Ericen_US
dc.date.accessioned2014-12-08T15:31:55Z-
dc.date.available2014-12-08T15:31:55Z-
dc.date.issued2013-08-13en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0073890en_US
dc.identifier.urihttp://hdl.handle.net/11536/22558-
dc.description.abstractNeuritogenesis is a process through which neurons generate their widespread axon and dendrites. The microtubule cytoskeleton plays crucial roles throughout neuritogenesis. Our previous study indicated that the amount of type II protein kinase A (PKA) on microtubules significantly increased upon neuronal differentiation and neuritogenesis. While the overall pool of PKA has been shown to participate in various neuronal processes, the function of microtubule-associated PKA during neuritogenesis remains largely unknown. First, we showed that PKA localized to microtubule-based region in different neurons. Since PKA is essential for various cellular functions, globally inhibiting PKA activity will causes a wide variety of phenotypes in neurons. To examine the function of microtubule-associated PKA without changing the total PKA level, we utilized the neuron-specific PKA anchoring protein MAP2. Overexpressing the dominant negative MAP2 construct that binds to type II PKA but cannot bind to the microtubule cytoskeleton in dissociated hippocampal neurons removed PKA from microtubules and resulted in compromised neurite elongation. In addition, we demonstrated that the association of PKA with microtubules can also enhance cell protrusion using the non-neuronal P19 cells. Overexpressing a MAP2 deletion construct which does not target PKA to the microtubule cytoskeleton caused non-neuronal cells to generate shorter cell protrusions than control cells overexpressing wild-type MAP2 that anchors PKA to microtubules. Finally, we demonstrated that the ability of microtubule-associated PKA to promote protrusion elongation was independent of MAP2 phosphorylation. This suggests other proteins in close proximity to the microtubule cytoskeleton are involved in this process.en_US
dc.language.isoen_USen_US
dc.titleMicrotubule-Associated Type II Protein Kinase A Is Important for Neurite Elongationen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0073890en_US
dc.identifier.journalPLOS ONEen_US
dc.citation.volume8en_US
dc.citation.issue8en_US
dc.citation.epageen_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.department生物資訊及系統生物研究所zh_TW
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.contributor.departmentInstitude of Bioinformatics and Systems Biologyen_US
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000323115800127-
dc.citation.woscount3-
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