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dc.contributor.authorHsieh, Yi-Heuien_US
dc.contributor.authorLai, Lee-Jeneen_US
dc.contributor.authorLiu, Shih-Jenen_US
dc.contributor.authorLiang, Keng S.en_US
dc.date.accessioned2014-12-08T15:32:05Z-
dc.date.available2014-12-08T15:32:05Z-
dc.date.issued2011-06-15en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2011.04.023en_US
dc.identifier.urihttp://hdl.handle.net/11536/22606-
dc.description.abstractThis work presents a rapid and sensitive method for detecting cancer cells at low concentration. In this method, two biomarkers of T-help cancer cells are detected simultaneously. One biomarker is conjugated with magnetic beads to separate T-help cell from the mixed cells and the other biomarker, associated with quantum dots, is used to detect fluorescence. The specific T-help cells can be quantified using the relationship between the QD fluorescence intensity and the cell frequency following magnetic separation. The intensity of fluorescence increases linearly with the frequency of T-help cells from 10(-7) to 10(-3), and neither B cells nor red blood cells interfere with the detection of T-help cells. Moreover, the total detection time is under 15 min, even though the frequency of specific T-help cells is as low as 5 x 10(-7). The numerous advantages of detecting specific cells at low concentration using the presented method include ease of preparation, low cost, fast detection, and high sensitivity. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectQuantum dotsen_US
dc.subjectMagnetic beaden_US
dc.subjectImmunoassayen_US
dc.subjectCancer cellen_US
dc.subjectFluorescenceen_US
dc.titleRapid and sensitive detection of cancer cells by coupling with quantum dots and immunomagnetic separation at low concentrationsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bios.2011.04.023en_US
dc.identifier.journalBIOSENSORS & BIOELECTRONICSen_US
dc.citation.volume26en_US
dc.citation.issue10en_US
dc.citation.spage4249en_US
dc.citation.epage4252en_US
dc.contributor.department電子物理學系zh_TW
dc.contributor.departmentDepartment of Electrophysicsen_US
dc.identifier.wosnumberWOS:000291904000045-
dc.citation.woscount10-
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