标题: | COLLAGEN VI PROTECTS AGAINST NEURONAL APOPTOSIS ELICITED BY ULTRAVIOLET IRRADIATION VIA AN AKT/PHOSPHATIDYLINOSITOL 3-KINASE SIGNALING PATHWAY |
作者: | Cheng, I. H. Lin, Y. -C. Hwang, E. Huang, H. -T. Chang, W. -H. Liu, Y. -L. Chao, C. -Y. 生物科技学系 生物资讯及系统生物研究所 分子医学与生物工程研究所 Department of Biological Science and Technology Institude of Bioinformatics and Systems Biology Institute of Molecular Medicine and Bioengineering |
关键字: | collagen VI;neuroprotection;UV;Akt/PI3K;NeurotoxQ |
公开日期: | 2-六月-2011 |
摘要: | Collagen VI, one of the extracellular matrix proteins, has been implicated in regulating cell proliferation and reducing apoptosis in several different systems. However, the role of collagen VI in the central nervous system remains unclear. In this manuscript, we demonstrated that upon ultraviolet (UV) irradiation, mouse primary hippocampal neurons specifically up-regulate the expression of Col6a1, Col6a2, and Col6a3 mRNA and secreted collagen VI protein. Augmentation of collagen VI mRNA and protein after UV irradiation may have a neuroprotective role as suggested by the fact that extracellular supplying soluble collagen VI protein, but not other collagen proteins, reduced UV induced DNA damage, mitochondria dysfunction, and neurite shrinkage. We also tried to determine the signaling molecules that mediate the protective effect of collagen VI via Western blot and inhibitor analysis. After collagen VI treatment, UV irradiated neurons increased phosphorylation of Akt and decreased phosphorylation of JNK. Inhibiting Akt/Phosphatidylinositol 3-kinases (PI3K) pathway diminished the protective effect of collagen VI. Our study suggested a potential protective mechanism by which neurons up-regulate collagen VI production under stress conditions to activate Akt/PI3K anti-apoptotic signaling pathway. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.neuroscience.2011.03.057 http://hdl.handle.net/11536/22792 |
ISSN: | 0306-4522 |
DOI: | 10.1016/j.neuroscience.2011.03.057 |
期刊: | NEUROSCIENCE |
Volume: | 183 |
Issue: | |
起始页: | 178 |
结束页: | 188 |
显示于类别: | Articles |
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