標題: Hypochlorous acid turn-on boron dipyrromethene probe based on oxidation of methyl phenyl sulfide
作者: Liu, Shi-Rong
Vedamalai, Mani
Wu, Shu-Pao
應用化學系
Department of Applied Chemistry
關鍵字: Hypochlorous acid;Fluorescent probe;Boron dipyrromethene;Bioimaging
公開日期: 24-十月-2013
摘要: A boron dipyrromethene (BODIPY)-based fluorometric probe, HCS, has been successfully developed for the highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of methyl phenyl sulfide. The reaction is accompanied by a 160-fold increase in the fluorescent quantum yield (from 0.003 to 0.480). The fluorescent turn-on mechanism is accomplished by suppression of photoinduced electron transfer (PET) from the methyl phenyl sulfide group to BODIPY. The fluorescence intensity of the reaction between HOCl and HCS shows a good linearity in the HOCl concentration range 1-10 mu M. The detection limit is 23.7 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HCS probe could be an efficient fluorescent detector for HOCl in living cells. (C) 2013 Published by Elsevier B.V.
URI: http://dx.doi.org/10.1016/j.aca.2013.09.018
http://hdl.handle.net/11536/22969
ISSN: 0003-2670
DOI: 10.1016/j.aca.2013.09.018
期刊: ANALYTICA CHIMICA ACTA
Volume: 800
Issue: 
起始頁: 71
結束頁: 76
顯示於類別:期刊論文


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