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dc.contributor.authorHo, Pei-Yinen_US
dc.contributor.authorChueh, Shih-Chiehen_US
dc.contributor.authorChiou, Shyh-Horngen_US
dc.contributor.authorWang, Shuo-Mengen_US
dc.contributor.authorLin, Wei-Chouen_US
dc.contributor.authorLee, I-Liangen_US
dc.contributor.authorYang, Hsin-Yien_US
dc.contributor.authorPeng, Hsiang-Chien_US
dc.contributor.authorLai, Ming-Kuenen_US
dc.date.accessioned2014-12-08T15:33:03Z-
dc.date.available2014-12-08T15:33:03Z-
dc.date.issued2013-10-01en_US
dc.identifier.issn1078-1439en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.urolonc.2012.01.015en_US
dc.identifier.urihttp://hdl.handle.net/11536/23016-
dc.description.abstractObjectives: AlphaB-crystallin (alpha B-crystallin), a small heat shock protein, has been reported to be involved in the growth, antiapoptosis, migration, and chemoresistance of human malignancies. Materials and methods: alpha B-crystallin expression in normal renal and clear cell renal cell carcinoma (ccRCC) tissues was examined with two-dimensional (2D) gel electrophoresis assays. Immunohistochemistry was conducted to determine the presence of alpha B-crystallin-positive tumor cells and staining intensity in 50 cases of ccRCC tissue samples. The association of alpha B-crystallin protein expression, clinicopathogic parameters and prognosis of ccRCC patients was also analyzed with Student's t-test and Kaplan-Meier analysis. Moreover, Western blot assays were performed to detect the protein expression of alpha B-crystallin in normal and tumor tissues and the alteration of cell cycle regulators in alpha B-crystallin-overexpressing cells. MTT (3[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide), BrdU, and transwell assays were performed to demonstrate the effects of aB-crystallin overexpression on cell growth, DNA synthesis and cell migration of ccRCC cells, respectively. Results: The results showed the up-regulation of alpha B-crystallin expression in ccRCC tissues. Overall survival of ccRCC patients was significantly correlated with alpha B-crystallin expression in tumor tissues. We found that aB-crystallin overexpression increased the expression of cyclin A and the incorporation of BrdU, which may be related to the enhancement of cell growth. Transwell analyses demonstrated that presence of alpha B-crystallin overexpression enhanced cell migration in ccRCC cells. Furthermore, rapamycin-resistance of tumor cells was induced when alpha B-crystallin was overexpressed. Conclusions: Our experimental findings highlight the importance of alpha B-crystallin in the tumor growth, migration, and target therapy-resistance of ccRCC cells. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectClear cell renal cell carcinomaen_US
dc.subjectalpha B-crystallinen_US
dc.subjectPrognostic factoren_US
dc.subjectProliferationen_US
dc.subjectMigrationen_US
dc.subjectDrug resistanceen_US
dc.titlealpha B-Crystallin in clear cell renal cell carcinoma: Tumor progression and prognostic significanceen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.urolonc.2012.01.015en_US
dc.identifier.journalUROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONSen_US
dc.citation.volume31en_US
dc.citation.issue7en_US
dc.citation.spage1367en_US
dc.citation.epage1377en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000325664300058-
dc.citation.woscount4-
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